MICROBIOLOGY Flashcards
(54 cards)
Grow in ambient air, which contains 21% O2 and small amount (0.03%) of CO2
Aerobes
Cannot grow in the presence of O2 and the atmosphere in anaerobe jars
Anaerobes
Grow under reduced O2 and increased CO2
Microaerophilic
Requires increased concentration of CO2 and approximately 15% O2
Capnophiles
Allow room (unsterilized) air to pass into the cabinet and around the material within, sterilizing only the air to be exhausted
Class I BSC
Self contained and 70% of the air is recirculated
Class IIA BSC
Air is discharged outside the building. It is selected if radioisotopes, toxic chemicals or carcinogens will be used
Class IIB BSC
Air coming into and going out of the cabinet is filter sterilized and the infectious material within is handled with rubber gloves that are attached and sealed to the cabinet
Class III
Classification of organisms according to hazard/ biosafety levels where no known potential for infecting the healthy people and are well defined and characterized
BSL-1
Classification of organisms according to hazard/ biosafety levels where most commonly being sought in clinical specimens and used in diagnostic, teaching and other laboratories
BSL-2
Classification of organisms according to hazard/ biosafety levels where recommended for the handling of material suspected of harboring organisms unlikely to be encountered ina routine clinical laboratory and for such organisms as M. Tuberculosis, C. Burnetii, the mold stages of systemis fungi and formsome other organisms when grown in quantities greater than that found in patient specimen
BSL-3
Classification of organisms according to hazard/ biosafety levels where exotic agents are considered high risk and cause life threatening disease
BSL-4
Chain of infection
Infectious organism / pathogen
Reservoir portal of exit
Mode of transmission
Portal of entry
Susceptible host
Safest method to ensure that no infective materials remain in samples or containers when disposed
Incineration
Used to sterilize biohazardous trash and heat-stable objects
Autoclave
Used to sterilize items such as glassware, oil, petrolatum or powders
Dry heat oven
Method of choice for antibiotic solutions, toxic chemicals, radioisotopes, vaccines and carbohydrates which are all heat sensitive
Filtration
Used for sterilizing disposables such as plastic syringes, catheters, or gloves before use
Ionizing radiotion
Most common chemical sterilant
Ethylene dioxide
Use of glutaraldehydeor peracetic acid
Cold sterilization
Mechanical action of rubbing the hands together and soaping undeneath fingernails
Most important part of handwashing
Round, smooth, convex, some strains are beta hemolytic; most colonies appear black or very dark purple; cultures smell of ammonium cyanide
Chromobacterium violaceum
Cause granulomatous disease in animals and have been associated with soft tissue infection in humans following animal bites
Actinobacillus spp.
Recovered from mixed populations of organisms and identified using the ethanol shock sore technique
Clostridium spp.
