Histoplasma

Question 1

2 important pathogenic species under Genus Histoplasma

Answer 1

Histoplasma capsulatum, H. farciminosum

Question 2

Perfect state of H. capsulatum

Answer 2

Ajellomyces capsulatus

Question 3

H. capsulatum causes

Answer 3

Histoplasmosis in humans and animals

Question 4

Morphology of H. capsulatum

Answer 4

Small, oval, yeastlike cells

Question 5

Stain used for H. capsulatum

Answer 5

Giemsa, Periodic Acid-Schiff (PAS), Hematoxylin and Eosin (H&E)

Question 6

At __°C, H. capsulatum produces pear-shaped microconidia

Answer 6

30°C

Question 7

Media used for H. capsulatum

Answer 7

Sabhi, Sabouraud dextrose agar, Mycobiotic agar

Question 8

At 30°C, H. capsulatum colonies are?

Answer 8

Fluffy, tan to whitish

Question 9

Yeast phase of H. capsulatum grows on what temperature?

Answer 9

37°C

Question 10

Yeast phase of H. capsulatum is grown on what agar?

Answer 10

Brain-heart infusion agar with glutamine

Question 11

Antigens of H. capsulatum

Answer 11

Histoplasmin, polysaccharide antigens

Question 12

Reservoir for H. capsulatum

Answer 12

Soil enriched with bird or bat droppings

Question 13

Carriers of histoplasmosis in Alabama, Arizona, Maryland, Oklahoma, Texas

Answer 13

Bats

Question 14

Transmission for H. capsulatum

Answer 14

Inhalation of airborne conidia

Question 15

Lesions for histoplasmosis are found in?

Answer 15

Lungs

Question 16

H. capsulatum most commonly infects this domestic animal

Answer 16

Dogs

Question 17

Histoplasmosis in this animal shows hepatomegaly, lymphadenopathy & ascites

Answer 17

Dogs

Question 18

This animal can transmit the histoplasmosis to humans through saliva, vomit, feces, and urine

Answer 18

Dogs

Question 19

Histoplasmosis in this animal shows ulcerative gastritis, hemorrhagic enteritis, splenomegaly, enlarged mesenteric lymph nodes

Answer 19

Guinea pigs

Question 20

Diagnosis for H. capsulatum

Answer 20

Smears on Periodic Acid-Schiff or Silver stains

Question 21

Culture for H. capsulatum

Answer 21

Emmon’s modified Sabouraud agar at 30°C

Question 22

Serologic tests for H. capsulatum

Answer 22

Complement fixation and Immunodiffusion

Question 23

H. capsulatum is sensitive to:

Answer 23

Amphotericin B, Ethyl vanillate, Ketoconazole, Miconazole, Nystatin, Sulfonamides

Question 24

Synonym for Histoplasma farciminosum

Answer 24

Blastomyces farciminosa, Cryptococcus farciminosus

Question 25

H. farciminosum is the causative agent for?

Answer 25

Epizootic lymphangitis of horses and mules

Question 26

Morphology of H. farciminosum in pus:

Answer 26

Double-contoured, yeastlike, oval

Question 27

Morphology of H. farciminosum in culture

Answer 27

Has hyphae and produces ascospores

Question 28

Media for H. farciminosum

Answer 28

Plain nutrient agar, nutrient broth, coagulated egg medium, serum medium

Question 29

Colony characteristic of H. farciminosum on solid media

Answer 29

Small, grayish-white granules, dry, become leaetherlike

Question 30

Colony characteristic of H. farciminosum on liquid media

Answer 30

Scanty, granular sediment

Question 31

Common lesion sites of H. farciminosum

Answer 31

Lower limb of horses, genitalia of mares

Question 32

Transmission for H. farciminosum

Answer 32

Direct or indirect contact, through wounds

Question 33

Immunity for H. farciminosum

Answer 33

Cell-mediated

Question 34

Diagnosis for epizootic lymphangitis (H. farciminosum)

Answer 34

Smears & Fluorescent antibody techniques (FAT)

Question 35

H. farciminosum is sensitive to?

Answer 35

Iodides and Ketoconazole