Idexx In House Analyzers

Question 0

What species can lasercyte hematology analyzer analyze samples from

Answer 0

Cat dog equine

Question 1

What can the laser cyte hematology analyzer do

Answer 1

Check red blood cells, Reticulocytes, white blood cells, platelet count

Question 2

Which white blood cells are granulocytes

Answer 2

All the Phils

Question 3

Which white blood cells are agranulocyte’s

Answer 3

All the cytes

Question 4

How does the lasercyte hematology analyzer work

Answer 4

Each cell goes through a laser beam. Light is scattered by the cell and measured by four detectors based on size, complexity, granularity, light absorption. The time of flight through the laser beam is also recorded

Question 5

What are the main components of the lasersight hematology analyzer

Answer 5

Reagent compartment containing sheath reagent bottle and waste bottle. Air filter, status light, loading tray

Question 6

In the CBC5R kit which tubes are contained

Answer 6

Purple top tubes, gray top tube, red top tubes, yellow top tubes

Question 7

What is inside the purple top tube

Answer 7

EDTA

Question 8

What’s inside the grey top tube

Answer 8

Quality beads, and new methylene blue stain to stain reticulocytes.

Question 9

What is inside the red top tube

Answer 9

Discard tube

Question 10

What is inside the yellow top tube

Answer 10

Automated enzymatic cleaner

Question 11

What does the sheath reagent do

Answer 11

Lyses red blood cells to isolate the white blood cells for analysis

Question 12

Go through the steps of analyzing a sample with the lasercyte

Answer 12

Collect a sample, Fill purple top that collect tubes with 0.5 to 1.5 mL. Invert eight times before collecting and running. Put sample and gray top tube in the analyzer. Enter patient info and run it.

Question 13

What are the different ways you can verify results from the laser sight analyzer

Answer 13

Dot Plot, blood smear

Question 14

How do you calibrate the laser sight analyzer

Answer 14

It’s done at the factory

Question 15

How is quality control run on the lasersight analyzer

Answer 15

Quality beads in reagent tubes and two different hemoglobin readings are done

Question 16

How does maintenance on the lasersight analyzer work

Answer 16

It’s automated. After every sample, every four hours, and every 24 hours it flushes and rinses. After every 20 samples the extensive enzymatic clean happens. Weekly the air filter must be cleaned, the system is rebooted and backed up, sheath reagent amount is checked, waste bottle is emptied

Question 17

What does the catalyst DX analyzer do

Answer 17

Chemistry analysis, electrolytes, UPC fructosamine phenobarbital.

Question 18

How does the catalyst DX analyzer work

Answer 18

By chemical reaction

Question 19

What are the main components of the catalyst DX analyzer

Answer 19

Touchscreen interface, sample drawer, waste drawer, maintenance access doors, tip and diluent drawer

Question 20

What are the pre-loaded clips you can put into the catalyst DX on a laser

Answer 20

Chem: 17 for canine, chem: 15 feline, Chem: 10 Preanesthetic, Lyte 4: electrolytes

Question 21

What are some other main components of the catalyst DX analyzer

Answer 21

lithium Heprin whole blood separator, pipettes, sample cups

Question 22

How do you prepare a whole blood sample for the catalyst DX analyzer

Answer 22

.7 mL in lithium heparin separator. Swirl five times

Question 23

How do you prepare a plasma sample for the catalyst DX analyzer

Answer 23

Whole blood in lithium heparin tube, mix, centrifuge. 300 µL in sample cup with no bubbles

Question 24

How do you prepare a serum sample for the catalyst DX analyzer

Answer 24

Dry or SST tube, allowed to Claude, centrifuge, 300 µL in sample cup with no bubbles

Question 25

How do you prepare a urine sample for the catalyst DX analyzer

Answer 25

Centrifuge, 300 µL of supernatant in sample cup

Question 26

How do you analyze the sample in the catalyst DX analyzer

Answer 26

Select patient data, load sample, load clip or slides with the Lyte 4 first, run, view results on catalyst or vet lab machine.

Question 27

How do you maintain the catalyst DX analyzer

Answer 27

Empty the waste drawer, clean the internal components

Question 28

How do you clean the internal components of the catalyst DX analyzer

Answer 28

Do it monthly and before quality control, lint free wipes with 70% alcohol

Question 29

When do you do the quality control

Answer 29

Monthly, after the analyzer has been moved to verify system performance

Question 30

How do you do a quality control sample

Answer 30

Client equals QC, patient equals lot number of QC clip, sample equals 300 µL vettrol solution, load QC clip and lyte 4 clip, compare results to pass fail values on QC log

Question 31

What does the vet lab station do

Answer 31

Integrates results into a single report, organizes results, reduces manual data entry, integrates with clinics computer system