Imaging

Question 1

Haemoatoxylin and Eosin Staining

Answer 1

Question 2

Immunohistochemistry method

Answer 2

Question 3

Advantages and Disadvantages of IHC

Answer 3

Question 4

Medican Applications of IHC

Answer 4

Question 5

What is immunoflourescence staining?

Answer 5

Similar to IHC, but uses fluorescent instead of enzymatic detection.

Immunofluorescence combines immunology (antibody-antigen binding) with fluorescence microscopy. When a fluorescent dye (fluorophore) attached to an antibody binds its target protein, it emits light when excited by a specific wavelength — allowing visualization of the protein in its native cellular environment.

Question 6

Immunofluorescence method

Answer 6

Question 7

Uses of immunofluorescence in medicine

Answer 7

Question 8

DNA and RNA In Situ Hybridisation

Answer 8

Question 9

COMET Technology

Answer 9

Question 10

Phenocycler tech

Answer 10

Question 11

GeoMx Digital Spatial Profiler

Answer 11

Question 12

Comparison Table for multiplex spatial proteomics with medicine application

Answer 12

✅ Multiplex – Analyze many molecules (RNA/proteins) at once
✅ Spatial – Preserve and study their location in the tissue
✅ Proteomics/Transcriptomics – Measure proteins (via antibodies) or mRNA (via probes)

Question 13

what are single cell spatial multiomics

Answer 13

Question 14

When to use what

Answer 14

Question 15

Tissue Slice Culture Models

Answer 15