Introduction to experimental models and nucleic acid methods

Question 1

What could be involved if one goes about observing DNA, RNA or proteins

Answer 1

Question 2

What is involved in experimental models?

Answer 2

Assessing the following :
- Accuracy
- Ethics
- Necessity
- Cost
- Numbers
- Usually 3 repeats

Always try use the smallest model (animal) possible, control (genetically identical, environment, age)

Question 3

2D vs 3D cell culturing

Answer 3

Question 4

What are organoids

Answer 4

Question 5

Pros of using single celled organisms in cluturing

Answer 5

Yeast or bacteria
- Easy to genetically modify
- Easy to grow and use in lab
- Cheap

Question 6

Using Humans

Answer 6

Issues :
- Ethically most protected species
- Genetically varied
- Highly variable environment
- Expensive
- Genetic modification close to impossible
- Few patients