Immunological Applications Flashcards
1
Q
How are polyclonal antibodies produced?
A
Through injecting antigens into animals. They are used as ‘machines’ in this instance.
2
Q
What are some applications used to isolate monoclonal antibodies?
A
ELISA test Immunofluorescence Confocal Microscopy Immunohistochemistry Immunoelectron microscopy FACS
3
Q
What is FACS used for?
A
Measure cell surface molecules
4
Q
What is the purpose of an ELISA test?
A
Test that uses antibodies and colour change to identify a substance.
5
Q
How can you select certain cells using antibodies?
A
Using paramagnetic particles and an iron wool mesh.
6
Q
Which techniques is suitable for identifying antigens through staining?
A
Immunohistochemistry
7
Q
Which techniques utilises the specificity of antibodies to identify cells?
A
Immunofluorescence. A light microscope is used to view.
8
Q
How is flow cytometry (FACS) used?
A
- Cytokines build up in cells by inhibiting protein export.
- Cell permeabilised and antibodies are introduced
- Specific cytokines bind and it shows what proteins are synthesized in the cell.
