Ingham-1: B and T cells, Abs Flashcards
(38 cards)
2 branches/parts of the adaptive immune system
Humoral and Cell-mediated
Which lymphocyte is responsible for humoral immunity?
B cell
Which lymphocyte is responsible for cell-mediated immunity?
T cell
What is a B cell antigen?
substance that binds Ab
What is a B cell antigen made of?
protein, carbohydrate or DNA
What is an epitope?
Portion of Ag recognized by Ab
What is polyclonal antisera?
collection of diff. Abs from serum that recognize diff. epitopes of the same Ag
What are monoclonal Abs?
Ab derived from a single B cell clone (all recognize the same epitope)
What 2 chains make up Ab? How many of each?
- heavy and light
- 2
What holds heavy and light chains together?
disulfide bonds
How many light chain genes do mice and humans have?
2
What are the 2 light chain genes called?
kappa and alpha
How many light chain alleles do mice and humans have?
4
How many heavy chain genes do mice and humans have?
1
How many heavy chain alleles do mice and humans have?
2
What are the 5 isotypes of heavy chains?
IgA, IgD, IgD, IgE, and IgM
What regions of the Ab bind Ag?
variable regions of heavy and light chains
Which chains have constant regions (heavy or light)?
both
What is the function heavy chain C-terminal constant region?
effector functions
What regions of the chains form Ig domain fold?
VH/VL and CH/CL (the ones that are covalently joined together)
What is the structure of Ig fold?
2 beta sheets forming a beta sandwich
What does CDR stand for?
complementary determining region
In terms of CDR, what forms the Ag-binding site? (for BCR)
CDRs of heavy and light chains
For TCR, what forms Ag-binding site (in terms of CDR)?
CDRs of alpha and beta chains
