Magor-2 Flashcards
(33 cards)
Function of TLR
- detect PAMPs and DAMPs
- produce antimicrobials, antivirals, cytokines; inflammation
3 parts of TLR
- has ligand-binding exterior domain
- membrane spanning domain
- interior Toll/IL-1R domain
Function of TLR Interior Toll/IL-1R domain (TIR)
- Interacts with TIR domains of other members of TLR signal transductions
- adaptor proteins bind here
What is the ligand-binding end of TLR made of?
- Leucine rich repeats (LRRs) (20-40 aa per repeat)
- has alpha helix and beta strand
What does TLR1 recognize?
- lipopeptides microbe (Gram -)
What does TLR2 recognize?
- Gram + bacteria
- parasite and fungus
What does TLR3 recognize?
dsRNA = virus
What does TLR4 recognize?
- LPS –> Gram -ve
- fungus
What does TLR5 recognize?
flagellin –> microbe
Example of TLR that binds directly to PAMPs
TLR5
What does TLR4 need to indirectly bind a PAMP?
CD14, MD-2 and LBP
What directs what type of cytokines made?
Adaptor molecules that bind the TIR domain
What do NLRs detect?
peptidoglycan and flagellin
What do RLRs detect?
ssRNA (-ve sense)
What can NLRs and RLRs do? (broadly)
amplify or block TLR signalling
What doe C-type lectin receptors (CLR) detect?
Carbohydrate PAMPs using CLR
4 types of pattern recognition families
TLR, CLR, RLR, and NLR
What molecules are normally on cell surface in healthy cells?
CD47 and CD31
2 major DAMPs
mitochondrial membrane and DNA
Who developed clonal selection theory?
Sir Macfarlane Burnet
Who proposed the idea of PAMPs and DAMPs?
- Charlie Janeway
- Polly Matzinger
What can TLR2 be paired with?
TLR1 and TLR6
What does TLR1/TLR2 recognize?
triacyl lipopeptide
What does TLR1/TLR6 recognize?
diacyl lipopetide
