Instrumentation

Question 0

5 examples of exciter lamps

Answer 0

• tungsten lamps
• halogen lamps
• xenon lamps
• mercury lamps
• vapor lamps

Question 1

Componenent of spectrophotometer that serves as light source

Answer 1

Exciter lamp

Question 2

Component of spectrophotometer that controls the amount of light that enters the monochromator

Answer 2

Entrance slit

Question 3

Component of spectrophotometer that produces light of specific wavelength from the exciter lamp

Answer 3

Monochromator

Question 4

3 types of monochromators

Answer 4

• prisms
• diffraction gratings
• interference filters

Question 5

Type of monochromator that is either a wedge-shaped glass, quartz or sodium chloride that disperses light to different angles of refraction due to different wavelengths

Answer 5

Prisms

Question 6

Type of monochromator that is composed of grooves (for refraction) and slits (for diffraction) in an aluminum surface of a flat piece crown glass; it refracts and diffracts light into different spectra

Answer 6

Diffraction gratings

Question 7

Component of diffraction gratings that is for refraction of light

Answer 7

Grooves

Question 8

Component of diffraction gratings that is for diffraction of light

Answer 8

Slits

Question 9

Type of monochromator that is composed of semitransparent silver films on both sides of a dielectric such as magnesium fluoride; they filter and allow transmission of 40-60% of incident light with a bandpass or bandwidth between 10-20 nm.

Answer 9

Interference filters

Question 10

Component of spectrophotometer that holds the sample to be analyzed. It can be soft glass (for acidic solution), borosilicate (for alkaline solution) or quartz or plastic

Answer 10

Cuvette or analytical cell

Question 11

Type of cuvette for acidic solution

Answer 11

Soft glass

Question 12

Type of cuvette for alkaline solution

Answer 12

Borosilicate

Question 13

4 types of cuvettes

Answer 13

• soft glass
• borosilicate
• quartz
• plastic

Question 14

Component of spectrophotometer that detects and collects the light energy transmitted and converts it into a current to produce a readable output

Answer 14

Photodetector

Question 15

Type of photodetector that has selenium coated with silver which serves as a negative electrode and an iron base which serves as a positive electrode

Answer 15

Barrier layer cells

Question 16

Type of photodetector that has a curved sheet of photosensitive material that serves as the cathode and a positively charged thin tube that serves as the anode; most widely used photodetector

Answer 16

Phototube

Question 17

Type of photodetector that has a cathode, anode and electron-multiplying dynodes

Answer 17

Photomultiplier tubes

Question 18

Type of photodetector that has detectors including photoresistor, photodiode and phototransistor that have replaced conventional phototubes in modern laboratory instruments

Answer 18

Semiconductor

Question 19

4 types of photodetectors

Answer 19

• barrier layer cells
• phototube
• photomultiplier tubes
• semiconductor

Question 20

5 components of the spectrophotometer

Answer 20

• exciter lamp
• entrance slit
• monochromator
• cuvette or analytical cell
• photodetector

Question 21

Used to protect cuvettes from unwanted light that may cause measurement errors

Answer 21

Light shield

Question 22

Shape of cuvettes that are used that are more preferable becuase they reduce incident light

Answer 22

Rectangular cuvettes

Question 23

2 types of filters used to check the wavelength calibration of the spectrophotometer

Answer 23

• holmium oxide filters

- Didymium filters

Question 24

Solution/filter that detects stray light Has a sharp cut-off wavelength for transmission

Answer 24

Nickel II sulfate

Question 25

Color of sodium flame

Answer 25

Yellow

Question 26

Color of potassium flame

Answer 26

Violet

Question 27

2 ions determined by atomic absorption spectrophotometer

Answer 27

Calcium and magnesium ion

Question 28

Instrument used to determine immunoglobulins

Answer 28

Nephelometer

Question 29

Measures amount of light scattered

Answer 29

Nephelometer

Question 30

Instrument where the analyte can absorb light with a short wavelength then emits the same light at a higher wavelength

Answer 30

Fluorometer

Question 31

Substances separated by electrophoresis

Answer 31

Proteins

Question 32

Separates proteins by electric current

Answer 32

Electrophoresis

Question 33

Method that separates amino acids

Answer 33

Chromatography

Question 34

Principle of chromatography

Answer 34

Difference of affinity to the mobile phase and the stationary phase

Question 35

Method used to determine gastric acidity

Answer 35

Volumetric/titrimetric method

Question 36

Condition where there is excessive secretion of gastric juice due to high levels of gastrin

Answer 36

Zollinger-Ellison syndrome

Question 37

Method used to determine total lipids in feces

Answer 37

Gravimetric method

Question 38

Instrument used to check the speed of the centrifuge

Answer 38

Tachometer/strobe light