Intro Flashcards

0
Q

What is phylogeny?

A

Measures evolutionary rships between organisms

Through genome sequencing

Determined by similarities and differences in gene base sequences

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1
Q

What is taxonomy?

A

Organism classification based on shared characteristics.

Hierarchical

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2
Q

Gram positive

A

Purple

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3
Q

Gram negative (colour)

A

Pink

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4
Q

Autotrophs (carbon source?)

A

From CO2

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5
Q

Heterotrophs (carbon source?)

A

From organic compounds

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6
Q

Which target sequence is used for phylogeny?

A

16s rRNA. (From small ribosomal subunit)

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7
Q

Why is said target sequence for phylogeny used?

A

In ALL Living things

& evolves SLOWLY. Good crinometer

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8
Q

Similarities between bacteria and archaea

A

Morphology

Binary fission

Motile by flagella

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9
Q

Why are archaea lysozyme insensitive

A

No beta 1-4 link in peptidoglycans

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10
Q

Archaea have peptidoglycans? T/F

A

False

Some have pseudopeptidoglycan

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11
Q

Archaea have different shaped 70s ribosome? T/F

A

True

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12
Q

Enterobacteriacae gram stain?

A

Negative

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13
Q

Enterobacteriacae oxidase test?

A

Oxidase negative

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14
Q

Mother to newborn E.coli strain

A

Ecoli K1

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15
Q

Parenteral route for infection

A

Directly onto tissues beneath skin

Insect bite/surgery/injections or wounds

16
Q

Example of Enterobacteriacae transferred through parenteral route

A

Yersinia Pestis

17
Q

Enterobacteriacae that takes a respiratory route for infection

A

Pneumonic plague

Yersina pestis

18
Q

Example of an endogenous Enterobacteriacae that causes UTI

A

UPEC

19
Q

Examples of Enterobacteriacae that cause GI tract infection through ingestion of contaminated food/water

A

Salmonella

Ecoli [O157:H7]

20
Q

O antigen is….

A

SOMATIC

Lipopolysaccharide

21
Q

H antigen is…

A

Flagella

22
Q

K antigen is…

A

Capsule

23
Q

Method used for serotyping

A

Agglutination in culture between antibody (on a panel) & antigen (on bacteria)

24
Q

What is pyelonephritis

A

Upper urethra infection

Kidney involvement
Fever. Serious.

25
Q

UPEC can be present as asymptomatic? T/F?

A

True

26
Q

Which Ecoli strains cause GI infections?

A

Enterovirulent Ecoli strains

Eg EHEC

27
Q

What is the major virulence factor in the Ecoli strain that causes neonatal meningitis?

A

K-1 antigen

Capsule

28
Q

Which Ecoli strain produces an AE lesion and shiva like toxins

A

EHEC

enterohaemorrhagic Ecoli

29
Q

Which Ecoli strain can cause haemorrhagic colitis?

A

EHEC

O157:H7

30
Q

Condition developed from EHEC which involves acute renal failure, & is often fatal.

A

Haemolytic ureamic syndrome

31
Q

What serves as the natural reservoir for EHEC?

A

Cattle