Intro to Hematology

Question 1

pathology

Answer 1

study of the causes, mechanisms, and effects of diseases, including the examination of body tissues, fluids, and organs

Question 2

clinical pathology

Answer 2

subspecialty of pathology that focuses on the diagnosis and monitoring of diseases based on lab testing on various bodily fluids

Question 3

sections of clinical pathology

Answer 3

hematology
chemistry
serology/immunology
tranfusion medicine
clinical microscopy
parasitology

Question 4

hematology

Answer 4

study of blood cells and their formation
CBC
blood smear evaluation

Question 5

CBC

Answer 5

RBC/WBC/platelet count
hemoglobin concentrations
PCV (packed cell volume)
differential WBC examination
calculation of absolute values
erythrocyte indices

Question 6

blood smear evaluation

Answer 6

can be part of CBC

Question 7

whole blood

Answer 7

RBC, WBC, platelets, plasma
55% plasma
45% RBC
<1% WBC/platelets

Question 8

plasma components

Answer 8

90% water and proteins
10% hormones/enzymes, nutrients, gases

Question 9

serum

Answer 9

liquid part of blood AFTER coagulation

Question 10

plasma

Answer 10

liquid cell-free portion of blood
obtained after treated with anticoagulant

Question 11

two classes of tubes

Answer 11

procoagulants
anticoagulants

Question 12

procoagulants

Answer 12

yield serum
red or tiger top

Question 13

red top

Answer 13

glass or plastic
glass - no additives
plastic - silicon coated

Question 14

tiger top

Answer 14

wax separator

Question 15

anticoagulant additives need to bind to

Answer 15

calcium

Question 16

heparin

Answer 16

antithrombin
reversible
RBC measurements

Question 17

EDTA

Answer 17

preferred
binds to calcium
irreversible
hematology

Question 18

citrates

Answer 18

preferred for coag panel
binds to calcium
reversible

Question 19

order of blood tubes

Answer 19

blue
red
tiger top
green
purple
gray

Question 20

sample volume depends on

Answer 20

amount of serum/plasma needed
hydration of animal

Question 21

enough blood to run tests how many times

Answer 21

3

Question 22

consider what in regards to sample size

Answer 22

patient size

Question 23

most common automated analyzers

Answer 23

impedance analyzers
laser-based flow cytometer

Question 24

5 types of WBCs

Answer 24

neutrophils
eosinophils
basophils
lymphocytes
monocytes

Question 25

impedance analyzers

Answer 25

based on electric currents/disruptions to currents uses adult size of cells

Question 26

laser-focused flow cytometry

Answer 26

focused laser beams evaluate size and density of solid components degree and angles allow for enumeration of cells dyes allow enumeration of mature vs immature RBCs

Question 27

histograms/scatter plots

Answer 27

provide visual rep of cells within a sample

Question 28

normal histogram - lymphocytes

Answer 28

50 - 100 fL

Question 29

normal histogram - mixed cell population

Answer 29

100 - 150 fL

Question 30

normal histogram - neutrophils

Answer 30

150 - 300 fL

Question 31

blood smear evaluation

Answer 31

different % of WBCs