investigating the structure of cells

Question 1

magnification equals what

Answer 1

size of image/size of object

Question 2

what 3 conditions should be placed on a cell fractionation solution

Answer 2

cold
isotonic
buffered

Question 3

why should a cell fractionation solution be cold

Answer 3

to reduce enzyme activity that could break down organelles

Question 4

why should a cell fractionation solution be isotonic

Answer 4

to prevent organelles bursting or shrinking as a result of OSMOTIC gain or loss of water

Question 5

why should a cell fractionation solution be buffered

Answer 5

to maintain a constant PH

Question 6

what is homogenation

Answer 6

where cells are broken up by a blender
releasing organelles
the resultant fluid is known as the homogenate

Question 7

what is ultracentrifugation process

Answer 7

tube of filtrate spun at low speeds in the ultracentrifuge
heaviest organelles fall to the bottom (the nuclei)
the heaviest form a pellet at the bottom
the supernatant is removed transferred and spun at a higher speed
the next heaviest organelles fall to the bottom (mitochondria)
process continued