Lab 1 Flashcards
(5 cards)
1
Q
what can influence the separation of organelles?
A
speed of centrifugation, density of separation media; density gradients, viscosity, pH ionic strength, etc
2
Q
the denser the object, the lower the speed needed to sediment
A
ya
3
Q
nomograms can be used for estimating RCF
A
ya
4
Q
SDH reaction and detection method
A
reduces FADH2–sol’n turns from blue to offwhite; when there is a small amount of activity white colour will appear at the bottom and move upwards
5
Q
LDH reaction and detection method
A
measure amount of NADH produced