Lab 8 Flashcards
(7 cards)
1
Q
what are the advantages of using a radioactive tag?
A
blots can be “stripped” of one Ab and reprobed with another
2
Q
what are the disadvantages of radioactive tags?
A
can be slow to obtain results; have to work with and dispose of radioactive materials
3
Q
advantages of alkaline phosphatase
A
obtains results relatively fast; can watch bands appear and control exposure; relatively inexpensive
4
Q
disadvantages of AP
A
background often higher than with other methods of detection; cannot strip and reprobe; bands tend to fade over time–photograph results
5
Q
advantages of HRP
A
very sensitive; obtain results relatively fast; low background; hard copy results on film
6
Q
disadvantages of HRP
A
relatively expensive
7
Q
what protein is being detected?
A
p85
