Lab 10-Chlamydophila

Question 1

Characteristics

Answer 1

Obligate intracellular bacteria

Propagate inside the vacuoles of the cytoplasms of the host cell
Cannot produce ATP on its own

Question 2

Propagation cycle

Answer 2

Elementary body (infectious form) -> reticulate body (inside cell)

Propagation cycle = 2-3 d

Question 3

Peptidoglycane layer ?

Answer 3

No, it is missing from the cell wall.

But genus specific LPS

Question 4

Habitat

Answer 4

Mucous membranes (gut, genitals, resp. tract)

Persistent sub clinical infection

Question 5

Morphology

Answer 5

0.2-0.4 um coccoid rods

Question 6

Is resistance of EB or RB better ?

Answer 6

Elementary body

Question 7

How do these bacteria spread ?

Answer 7

EB enter host cell, gets transformed into RB (propagation). Then they turn back into EB, the cell is so destroyed it will lyse, EB will escape the cell.

Question 8

RB size ?

Answer 8

Bigger than EB

Question 9

Staining

Answer 9

Gram -
Stamp staining, Giemsa

Stamp: 
Fixation over benzen burner
1% acetic acid treatment 
Rinsing with H2O
3% diluted malachite-green (0.5-1 min)
Rinsing, drying 

Background will be blue/greenish
Chlamydia will be pink/redish

Question 10

Culture

Answer 10

In living cells only
5-7d old embryonated egg
Tissue culture (McCoy, L929, BHK, Vero)
Animals

McCoy= chlamydia is green

Question 11

Antigens

Answer 11

Genus specific
-LPS
Species specific
- protein AGs

Question 12

Resistance

Answer 12

Weak

EB more resistant

Survive in dried faeces, fetal membranes, dust 1-3w

Question 13

Species

Answer 13

C. Trachomatis (human)
C. Psittaci (zoonotic)
C. Abortus
C. Felis (cat)
C. Pecorum (cattle)
C. Pneumoniae (human)

Question 14

C. Trachomatis cause?

Answer 14

Chronic purulent conjunctivitis in human
Flies are vectors !

Lymphogranuloma venereum- inflammation of urethra, vaginitis, enlargement of reg. Lymph nodes
(Sexual transmitted disease)

Question 15

C. Psittaci cause?

Answer 15

Birds: Parrot disease,parrot fever

Mammals, humans: conjunctivitis, diarrhea, respiratory infection

Humans: influenza like symptoms, headaches, pneumonia

Occupational disease: zoo workers, slaughterhouses workers, vets

Question 16

C. Abortus cause ?

Answer 16

Abortion in ewe, goat, cattle, swine

Question 17

C. Felis

Answer 17

Cat conjunctivitis (30% of cases)

Question 18

C. Pneumoniae cause ?

Answer 18

Human pneumonia

Question 19

Can cause in pigeons ?

Answer 19

Ornithosis

Question 20

Common name of disease ?

Answer 20

Chlamydiosis