Lab 3- Staphylococcus

Question 1

Habitat

Answer 1

Skin, mucous membrane, food, plant, soil and water

Question 2

Morphology

Answer 2

Coccus

Bunch of grapes

Question 3

Staining

Answer 3

Gram positive

Question 4

Steps of gram staining

Answer 4

Crystal violet 3-5 min 
Lugol solution 1-1,5 min 
96% alcohol, some drops 
Water 
Fuchsin 0,5-1 min 
Water
Drying

Question 5

Culture

Answer 5

Nutrient agar, broth

Question 6

Pigment

Answer 6

Golden, white

Question 7

Hemolysis

Answer 7

B hemolysis (sometimes double)
No hemolysis

Question 8

Selective culture

Answer 8

10% NaCl-mannitol-phenol red agar

Må-tellurite-glycine-pyruvic acid - egg (Baird-Parker agar)

Question 9

Biochem

Answer 9

Catalase +
Oxidase -
Fermentative

Extracellular enzymes:
Coagulase, fibrinolysin, hyaluronidase

Surface proteins: protein-A

Toxins:
haemolysis, leucocidins, enterotoxins, dermotoxin (exfolitative toxin)

Question 10

Antigens

Answer 10

Complex

Question 11

Resistance

Answer 11

Good

Question 12

Pathogenicity

Answer 12

Local suppuration, abscesses, arthritis, mastitis, metritis, dermatitis, septicaemia

Question 13

Species

Coagulase positive

Answer 13

Extracellular enzymes 
Toxins 
Hemolysis
Mannitol + 
Facultative pathogens

Question 14

Species

Coagulase negative

Answer 14

Less extracellular enzymes 
Toxin prod. Not typical 
No hemolysis 
Mannitol -
Generally saprophytes, sometimes subclinical infections

Question 15

Coagulase positive species:

Answer 15

S. Aureus subspecies aureus
S. Aureus subspecies anaerobius
S. Pseudointermedius
S. Intermedius

Question 16

What species does s. Aureus subspecies aureus infect?

Answer 16

Cattle, swine, horse, sheep, dog and poultry
(Biotype A-E in different hosts)

MRSA: methicillin resistant s. Aureus

Question 17

What species does s. Aureus subspecies anaerobius target and what disease does it cause?

Answer 17

Sheep

Morel disease

Question 18

Target species and disease s. Pseudointermedius ?

Answer 18

Dog, cat

Dermatitis, otitis externa

Question 19

Target species s. Intermedius

Answer 19

Dog, horse, birds: mucous membranes

Saprophyte

Question 20

Coagulase negative species:

Answer 20

S. Epidermis 
S. Haemolyticus
S. Hyicus 
S. Gallinarum 
S. Equorum 
S. Felis

Question 21

S. Epidermis target species and disease?

Answer 21

Horse and dog

Wound infection

Question 22

S. Hemolyticus found in ?

Answer 22

Milk

Question 23

S. Hyicus

Answer 23

A part of the strain is coagulase positive

Exudative epidermitis

Question 24

S. Gallinarum cause?

Answer 24

Dermatitis

Question 25

S. Equorum cause?

Answer 25

Dermatitis

Question 26

S. Felis cause?

Answer 26

Dermatitis

Question 27

Habitat Micrococcus?

Answer 27

Environment, mucous membranes, food, skin

Question 28

Morphology Micrococcus?

Answer 28

Coccus

Question 29

Biochem Micrococcus?

Answer 29

Glucose utilization is aerobic or missing

Question 30

Pathogenicity Micrococcus?

Answer 30

Saprophyte