LAB 2

Question 1

average volume of an erythrocyte

Answer 1

MEAN CELL VOLUME

Question 2

unit of measurement for Mean cell volume

Answer 2

femtoliters (fL), or 10-15 L

Question 3

mcv calculation

Answer 3

MCV = [HCT (%) × 10]/RBC (×1012/L)

Question 4

reference range for mcv

Answer 4

80-100 fL

Question 5

<80 fL

Answer 5

Microcytic

Question 6

> 100 fL

Answer 6

Macrocytic`

Question 7

❖ average weight (content) of hemoglobin in a red blood cell

Answer 7

MEAN CELL HEMOGLOBIN

Question 8

MEAN CELL HEMOGLOBIN unit of measurement

Answer 8

picograms (pg), or 10-12 g

Question 9

mch calculation

Answer 9

MCH = HGB (g/dL) × 10/RBC (×1012/L)

Question 10

reference range mch

Answer 10

26-32 pg

Question 11

average concentration of hemoglobin in each individual red
blood cell

Answer 11

MEAN CELL HEMOGLOBIN CONCENTRATION

Question 12

MCHC Unit of measurement

Answer 12

Unit of measurement: grams per deciliter

Question 13

mchc calculation

Answer 13

MCHC = HGB (g/dL) × 100/ HCT (%)

Question 14

reference range mchc

Answer 14

32-36 g/dL

Question 15

normal
MCV

Answer 15

Dimorphic population of RBCs

Question 16

↑ MCV

Answer 16

↑ reticulocytes

Question 17

MCHC = 36-38 g/dL

Answer 17

check for spherocytes

Question 18

MCHC > 38 g/dL

Answer 18

analytical problem →
check hemoglobin value

Question 19

Markedly ↑ MCHC

Answer 19

presence of cold
agglutinins

Question 20

❖ Markedly ↑ MCHC → presence of cold
agglutinins
* Corrective action:

Answer 20

incubate specimen at
37° C for 15 minutes prior to analysis

Question 21

mcv: <80 fL
mchc: <32 g/dL

Answer 21

Microcytic; hypochromic

Question 22

Microcytic; hypochromic

Answer 22

Iron deficiency anemia, anemia of inflammation, thalassemia, Hb E disease and
trait, sideroblastic anemia

Question 23

mcv: 80-100 fL
mchc: 32-36 g/dL

Answer 23

Normocytic; normochromic

Question 24

Normocytic; normochromic

Answer 24

Hemolytic anemia, myelophthisic anemia, bone marrow failure, chronic renal disease

Question 25

mcv: >100 fL mchc: 32-36 g/dL

Answer 25

Macrocytic; normochromic

Question 26

Macrocytic; normochromic

Answer 26

Megaloblastic anemia, chronic liver disease, bone marrow failure, myelodysplastic syndrome

Question 27

It assesses erythropoietic activity of the bone marrow.

Answer 27

RETICULOCYTE COUNT

Question 28

RETICULOCYTE COUNT principle (1)

Answer 28

Blood sample + supravital stain (NMB, BCB) → dye- ribonucleoprotein complex (dark blue filamentous strands)

Question 29

retic count principle (2)

Answer 29

Reticulocyte → any nonnucleated RBC containing 2 or more particles of blue-stained, granulofilamentous material

Question 30

retic count specimen

Answer 30

❖ Whole blood anticoagulated with EDTA ❖ Capillary blood

Question 31

retic count reagent

Answer 31

New methylene blue (recommended) OR Brilliant cresyl blue

Question 32

retic counting

Answer 32

rountine light microscope method calibrated miller disc method

Question 33

routine light microscope method formula

Answer 33

Reticulocytes (%) = number of reticulocytes × 100/1000 ▪ OR Reticulocytes (%) = number of reticulocytes × 0.1

Question 34

permits a rapid survey of erythrocytes; provides a more precise reticulocyte count ▪ composed of 2 squares | area of smaller square: 1/9 the area of the larger square

Answer 34

Miller Ocular Disc

Question 35

in calibrated miller disc method what are counted in smaller square

Answer 35

RBCs

Question 36

in calibrated miller disc method what are counted in larger square

Answer 36

Reticulocytes

Question 37

calibrated miller disc method calculation

Answer 37

Reticulocytes (%)= No. of reticulocytes in square A (large square) × 100/ No. of RBCs in square B (small square) × 9

Question 38

REFERENCE RANGE MICROHEMATOCRIT

Answer 38

0.5 % – 2.5 %

Question 39

↑ amount of blood sample added to the staining solution

Answer 39

Anemic

Question 40

↓ amount of blood sample added to the staining solution

Answer 40

Polycythemic

Question 41

↓ reticulocyte count

Answer 41

Improperly mixed solution prior to smear preparation ↑ glucose level

Question 42

→ ↑ retic count

Answer 42

Artifacts (moisture in the air or poor drying of slide) can be mistaken as retics Presence of RBC inclusions: Heinz, Howell-Jolly, and Pappenheimer bodies

Question 43

disorders decreased reticulocytes

Answer 43

Aplastic anemia Aplastic crises of hemolytic anemia Chemotherapeutic or radiation-induced hypoproliferation Pernicious anemia Decreased erythropoiesis

Question 44

increased reticulocytes disorders

Answer 44

Blood loss Crisis associated with hemolytic anemia Subsequent to treatment of pernicious anemia, folic acid deficiency, or iron deficiency

Question 45

It is the actual number of reticulocytes in 1 liter (L) or 1 microliter (μL) of blood.

Answer 45

ABSOLUTE RETICULOCYTE COUNT

Question 46

formula for ABSOLUTE RETICULOCYTE COUNT

Answer 46

Absolute Reticulocyte count = [reticulocytes (%) × RBC count]/100

Question 47

Absolute Reticulocyte count reference range

Answer 47

20 × 10 9 /L – 115 × 10 9 /L

Question 48

CRC (%) =

Answer 48

Reticulocytes (%) × [Hct (%)/45 %]

Question 49

crc depends on

Answer 49

degree of anemia

Question 50

crc of normal hematocrit

Answer 50

approximately 1%

Question 51

crc of hematocrit value of 35 %

Answer 51

2-3%

Question 52

crc of hematocrit value

Answer 52

3-5%

Question 53

It is a general indicator of the rate of erythropoiesis increase above normal in anemias.

Answer 53

RETICULOCYTE PRODUCTION INDEX/SHIFT CORRECTION

Question 54

normal maturation time (2 days) in the BM → normal (nonshift) reticulocytes in the PB (1 day) → mature RBCs

Answer 54

normal

Question 55

↓ maturation time (<2 days) in the BM → shift reticulocytes in the PB (>1 day) → mature RBCs

Answer 55

Intense erytropoietic stress

Question 56

reticulocyte count is falsely ↑

Answer 56

polychromasia is present