Lab 3 Spectrophotmeter

Question 1

methods are widely used to quantify concentration of biological materials

Answer 1

photometric

Question 2

enables accurate determination of very small quantities

Answer 2

sensitivity

Question 3

physical basis to determine the physical characteristsics of chemicals to absorb what… at specific …. depending on atomorc structure

Answer 3

light ,wavelength

Question 4

lesser light leaves the solution

Answer 4

true, because most are absorbed

Question 5

uniform absorbing solution proportion of light passing is called

Answer 5

transmittance

Question 6

transmittance expressed as

Answer 6

T-l/lo

Question 7

light absorbed by substance

Answer 7

absorbance

Question 8

light not absorbed

Answer 8

transmittance

Question 9

absorbance unit

Answer 9

optical density OD - absorbance per unit length

Question 10

absorbance / thickness fo sample

Answer 10

OD

Question 11

transmittance and absorbance are

Answer 11

linear interdependent

Question 12

OD =

Answer 12

log10(1/T)

Question 13

transmittance ranges from

Answer 13

1-100%

Question 14

absorbance ranges

Answer 14

0-infinity

Question 15

states that absorbance is proportional to the composition of the concentration of the substance and the length of material that the light travels through

Answer 15

beer lamber law

Question 16

exceptions are

Answer 16

ionizing compounds, out of range concentration (high)

Question 17

amount of light transmitted at any given wavelength is a function of

Answer 17

concentration

Question 18

relationship b/t concentration and light

Answer 18

straight line

Question 19

simplified spectrophotometer use filters to segregate specific wavelengths

Answer 19

colorimeter

Question 20

measures turbidity

Answer 20

turbidometer

Question 21

measures concentration by the flourescence of materials

Answer 21

fluorometer

Question 22

measures concentration by the excitation of materials by heat and flame with consequent development of color

Answer 22

flame photometer

Question 23

when extremes are reached it is called

Answer 23

topping or bottoming out

Question 24

colorimeter usable range

Answer 24

0.01-1.0 OD units

Question 25

dilution factor =

Answer 25

total end volume/ volume of stock solution (used)

Question 26

final concentration=

Answer 26

original concentration/dilution factor