Lab 5: How Enzymes Function

Question 1

What symbol represents the change that occurs in a chemical reaction?

Answer 1

an arrow, —–>

Question 2

Are the substrates in an enzymatic reaction reactant or products?

Answer 2

reactants

Question 3

What is the name of the location on an enzyme where its substrate or substrates bind?

Answer 3

active site

Question 4

What cellular organelle contains catalase and is present in all plant and animal organs?

Answer 4

peroxisome

Question 5

What did measuring the bubble column heights in all the experimental procedures indicate?

Answer 5

a reaction

Question 6

What purpose did the tube containing water and hydrogen peroxide serve in the catalase activity experimental procedure?

Answer 6

negative control

Question 7

What word describes the loss of an enzyme’s original shape and ability of its active site to function?

Answer 7

denatures

Question 8

What happens to enzyme activity if the temperature increases tightly?

Answer 8

increases

Question 9

What did the negative control tube lack when the effect of enzyme concentration was studied?

Answer 9

a catalase

Question 10

If cells produce more catalase, will there be more or less water and oxygen produced from the breakdown of hydrogen peroxide by catalase?

Answer 10

more

Question 11

What word describes the pH at which the speed of a reaction involving an enzyme occurs best?

Answer 11

optimal

Question 12

What does a shift in pH (too high or too low) do to an enzyme that will influence its acitivity?

Answer 12

reduce activity

Question 13

In the Experimental Procedure on the effect of pH on enzyme activity, which tube contained water with an acidic pH?

Answer 13

tube 1

Question 14

Since enzymes are specific for certain substrates and often have similar names, what is the name of the enzyme used in tube 3 of the catalase activity experiment that facilitates the breakdown of sucrose?

Answer 14

sucrase

Question 15

What occurs during a degradation reaction?

Answer 15

the substrate is broken down to the products

Question 16

What occurs during a synthesis reaction?

Answer 16

substrates are joined to form a product

Question 17

When do enzymes denature?

Answer 17

enzymes begin to break down when the temperature increases

Question 18

What happens when you increase the amount of enzyme per the amount of substrate?

Answer 18

it is more likely that substrates will find the active site of an enzyme and a reaction will take place