lab 6

Question 1

Different microorganisms are capable of different processes. Give examples

Answer 1

Some are fermenters

Some can use photosynthesis

Some are capable of degrading non-organic material

Some ingest smaller microorganisms

Question 2

We are able to differentiate microorganisms based on their

Answer 2

ability to perform different biochemical processes

Question 3

Some microorganisms ferment carbohydrates
Remember

Answer 3

MacConkey agar and EMB agar

Question 4

Common fermentable sugars are for Carbohydrate fermentation

Answer 4

Sucrose
Dextrose (D-glucose)
Lactose

Question 5

Microorganisms breakdown carbohydrates through and are then recycled using

Answer 5

glycolysis in order to make ATP

They then recycle electron carriers using fermentation

Question 6

Some microorganisms are very ______ about what sugars they use for ferment

Answer 6

picky

Question 7

What can be used to identify microorganisms

Answer 7

We can use the ability to ferment certain sugars to help identify microorganisms

Question 8

What are the steps to the carbohydrate fermentation test?

Answer 8

1. Culture microorganisms in medium containing phenol red (a pH indicator) and Sucrose, Dextrose, or Lactose
2. Incubate overnight at 37° C
3. Organisms that can ferment carbohydrates will produce acid, turning the medium-yellow
4. Some microorganisms will also produce gas as a byproduct of fermentation. This will cause an air bubble in the tube

Question 9

What color means a positive carbohydrate fermentation test?

Answer 9

Yellow

Question 10

what indicator is used for the arbohydratest?

Answer 10

phenol red
Sucrose, dextrose, lactose

Question 11

What does a positive fermentation test with gas looks like?

Answer 11

air bubbles in the tube, tube would look yellow