lab 7

Question 1

IMViC Tests is used to

Answer 1

Help identify enteric bacteria (bacteria found in the gut)

Question 2

Enteric bacteria can be

Answer 2

Pathogens
Occasional pathogens
Normal gut flora

Question 3

IMViC Tests Consists of 4 tests:

Answer 3

Indole
Methyl Red
Vogues-Proskauer
Citrate Utilization

Question 4

Tests of indole, a product of

Answer 4

of tryptophan hydrolysis, is present

Question 5

Indole Test:Bacteria are grown in _____, which contain

Answer 5

SIM agar
trypotphan

Question 6

Indole Test:Addition of Kovak’s reagent will turn indole

Answer 6

cherry red

Question 7

Kovak’s reagent =

Answer 7

, p-dimethylaminobenzaldehye, and HCl

Question 8

Methyl Red TestMethyl Red is a pH indicator

Answer 8

Red for solutions with a pH < 6.2
Yellow for solutions with pH > 6.2

Question 9

The use of Methyl red is a test for

Answer 9

glucose fermentation

Question 10

Methyl Red TestONLY tests for acidic end products of

Answer 10

fermentation

Question 11

Vogues-Proskauer Test

Answer 11

Some organisms ferment glucose, but produce nonacidic or neutral end products

Question 12

Some organisms ferment glucose, but produce nonacidic or neutral end products Example:

Answer 12

production of acetylmethylcarbinol by E. aerogenes

Question 13

Addition of Barrit’s reagent will turn the solution

Answer 13

RED if non-acidic fermentation has occurred
Barrit’s reagent = α-naphthol + 4%KOH