HYDROLYSIS TEST

Question 1

What is the result of a positive hydrolysis test

Answer 1

When these enzymes break down these macromolecules, they produce water.

Question 2

We can determine whether or not a microorganism produces enzymes for a positive hydrolysis test by whether or not

Answer 2

they can liquify media solidified using these macromolecules

Question 3

What are the steps to a hydrolsysis test

Answer 3

1. Culture sample on a starch-agar plate with your microorganism
2. Incubate overnight at 37° C
3. Flood plate with Gram’s iodine for 30 seconds. Pour off excess
4. Examine cultures. Cultures which can digest starch will appear clear around the streak. This is because iodine dyes starch.

Question 4

Cultures which can digest starch will appear ________. This is because_______

Answer 4

Will appear clear around the streak
This is because iodine dyes starch.

Question 5

What are the steps to a gelatin hydrolysis test

Answer 5

1. Make a stab culture in nutrient gelatin tube
2. Incubate overnight at 37° C
3. Examine cultures. Cultures which can digest gelatin will have liquified the media.

Question 6

Triple Sugar-Iron Agar Test: This test differentiates between different types:
refering to bacteria

Answer 6

Enterobacteriaceae

Question 7

What are Enterobacteriaceae

Answer 7

are gram-negative bacilli that can ferment glucose

Question 8

Differentiation between Enterobacteriaceae is possible by examining

Answer 8

differences in carbohydrate fermentation patterns and sulfide production

Question 9

TSI medium contains

Answer 9

3 carbohydrate sugars (dextrose, sucrose, and lactose),
sodium thiosulfate,
and phenol red

Question 10

Triple Sugar-Iron Agar Test Results: Red slant, yellow butt, + or – gas production

Answer 10

Only glucose fermentation occurred

Question 11

Triple Sugar-Iron Agar Test Results: Yellow slant, red butt, + or – gas production

Answer 11

Sucrose and/or lactose fermentation occurred

Question 12

Triple Sugar-Iron Agar Test Results: Red slant, red butt, no gas

Answer 12

No carbohydrate fermentation has occurred.

Question 13

Triple Sugar-Iron Agar Test Results: Black butt

Answer 13

Organisms can use sulfur.
Result of H2S production reacting with iron in thiosulfate

Question 14

Hydrolysis test: Some bacteria produce enzymes that can digest

Answer 14

extracellular materials

Question 15

Hydrolysis test: Extracellular materials include

Answer 15

Casein
Gelatin
Starch
Lipids