HYDROLYSIS TEST Flashcards

1
Q

What is the result of a positive hydrolysis test

A

When these enzymes break down these macromolecules, they produce water.

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2
Q

We can determine whether or not a microorganism produces enzymes for a positive hydrolysis test by whether or not

A

they can liquify media solidified using these macromolecules

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3
Q

What are the steps to a hydrolsysis test

A
  1. Culture sample on a starch-agar plate with your microorganism
  2. Incubate overnight at 37° C
  3. Flood plate with Gram’s iodine for 30 seconds. Pour off excess
  4. Examine cultures. Cultures which can digest starch will appear clear around the streak. This is because iodine dyes starch.
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4
Q

Cultures which can digest starch will appear ________. This is because_______

A

Will appear clear around the streak
This is because iodine dyes starch.

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5
Q

What are the steps to a gelatin hydrolysis test

A
  1. Make a stab culture in nutrient gelatin tube
  2. Incubate overnight at 37° C
  3. Examine cultures. Cultures which can digest gelatin will have liquified the media.
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6
Q

Triple Sugar-Iron Agar Test: This test differentiates between different types:
refering to bacteria

A

Enterobacteriaceae

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7
Q

What are Enterobacteriaceae

A

are gram-negative bacilli that can ferment glucose

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8
Q

Differentiation between Enterobacteriaceae is possible by examining

A

differences in carbohydrate fermentation patterns and sulfide production

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9
Q

TSI medium contains

A

3 carbohydrate sugars (dextrose, sucrose, and lactose),
sodium thiosulfate,
and phenol red

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10
Q

Triple Sugar-Iron Agar Test Results: Red slant, yellow butt, + or – gas production

A

Only glucose fermentation occurred

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11
Q

Triple Sugar-Iron Agar Test Results: Yellow slant, red butt, + or – gas production

A

Sucrose and/or lactose fermentation occurred

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12
Q

Triple Sugar-Iron Agar Test Results: Red slant, red butt, no gas

A

No carbohydrate fermentation has occurred.

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13
Q

Triple Sugar-Iron Agar Test Results: Black butt

A

Organisms can use sulfur.
Result of H2S production reacting with iron in thiosulfate

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14
Q

Hydrolysis test: Some bacteria produce enzymes that can digest

A

extracellular materials

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15
Q

Hydrolysis test: Extracellular materials include

A

Casein
Gelatin
Starch
Lipids

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