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Bacteriology > Lab 7. Pasteurella > Flashcards

Lab 7. Pasteurella Flashcards

(37 cards)

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1
Q

Pasteurellaceae genera:

A

Genera:

  • Pasteurella
  • Mannheimia
  • Bibersteinia
  • Actinobacillus
  • Haemophilus
  • Avibacterium
  • Histophilus
  • Gallibacterium

Other genera resembling Family Pasteurellaceae:

  • Ornithobacterium
  • Riemerella
  • Taylorella
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2
Q

Pasteurellaceae

  • Habitat, morphology, staining and culture:
A

Habitat:

  • mucous membranes (respiratory way, genitals, alimentary tract)

Morphology:

  • 0.5-2 um coccoid rods

Staining:

  • Gram negative
  • Bipolar staining

Culture:

  • fastidious (yeast extract, blood, pH-susceptibility)
  • aerobic - facultative anaerobic
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3
Q

Pasteurellaceae

  • Biochemistry, antigens, resistance and pathogenicity
A

Biochemistry:

  • catalase +, oxidase -, fermentative,
  • toxin production by some species

Antigens:

  • cell wall antigens
  • capsule antigens

Resistance:

  • weak

Pathogenicity:

  • facultative pathogens
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4
Q

Pasteurella

  • Culture:
A

Fastidious

  • blood agar
  • yeast extract
  • pH susceptibility

Colony types

  • M-S-R colonies

Smell

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5
Q

Pasteurella

  • Biochemistry
A

Catalase +, oxidase -, fermentative

cytotoxin production

  • P. multocida D:

–> dermonecrotoxin: damage of the osteoblasts

  • P. multocida subspecies:

–> biochemical differences

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6
Q

Pasteurella

  • Antigens, resistance
A

Antigens:

  • P. multocida
  • Cell wall:

–> 16: Heddleston system

–> 11: Namioka system

  • capsule:

–> A, B, D, E, F

Resistance: weak

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7
Q

Pasteurella

  • Pathogenicity:
A

P. multocida A, D:

  • ruminants, swine pneumonia
  • rabbit septicaemia, pneumonia
  • fowl cholera

P. multocida D: atrophic rhinitis of swine

P. multocida B, E: haemorrhagic septicaemia

P. pneumotropica: lab. rodents pneumonia, dog, cat oral cavity

P. aerogenes: alimentary tract of swine, abortion

P. canis, P. dagmatis, P. stomatis: dog, cat oral cavity, bite

P. caballi: horse respiratory infection

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8
Q

Mannheimia, Bibersteinia:

  • Culture, resistance
A

Culture:

  • fastidious

–> blood agar, yeast extract, pH-susceptibility

–> b- hemolysis

Resistance: weak

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9
Q

Mannheimia, Bibersteinia

  • Biochemistry, antigens
A

Biochemistry:

  • catalase +, oxidase +, fermentative
  • leukotoxin production: damage of macrophages and endothelial cells

Antigens:

  • cell wall (cannot be used in diagnostics)
  • capsule (M. haemolytica and B. trehalosi have common system)

–> M. haemolytica 12 types

–> B. trehalosi 4 types

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10
Q

Mannheimia, Bibersteinia:

  • pathogenicity
A

M. haemolytica:

  • ruminants pneumonia
  • lamb septicaemia
  • mastitis, arthritis

B. trehalosi:

  • acute systemic pasteurellosis of feedlot lambs
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11
Q

Actinobacillus

  • Species
A
  • A. lignieresii
  • A. equuli
  • A. suis
  • A. seminis
  • A. ureae
  • A. pleuropneumoniae
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12
Q

A. lignieresii

  • habitat, morphology, staining, culture
A

Habitat:

  • cattle, sheep oral cavity, rumen, gut

Morphology:

  • 1-1.2 um rods, filaments
  • sulphur granules in lesions (like: actinomycosis)

Staining:

  • gram-negative, bipolar

Culture:

  • aerobic, facultative anaerobic
  • poor growth on nutrient agar
  • sticks to the agar
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13
Q

A. lignieresii

  • Biochemistry, antigens, resistance, pathogenicity
A

Biochemistry:

  • catalase +, oxidase +, fermentative

Antigens:

  • cell wall antigens: 6 serogroups

Resistance: weak

Pathogenicity:

  • cattle, sheep, swine actinobacillosis
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14
Q

A. equuli

  • Habitat, morphology, staining, culture
A

Habitat:

  • horse, swine alimentary tract, tonsils, respiratory tract

Morphology:

  • 1-2 um rod, filaments

Staining:

  • gram negative

Culture:

  • large, viscous colonies
  • some strains can haemolyse
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15
Q

A. equuli

  • resistance, subspecies, pathogenicity
A

Resistance: weak

Subspecies, pathogenicity:

  • A. equuli subsp. equuli
  • A. equuli subsp. haemolyticus
  • both subspecies cause the same disease

–> foal: septicaemia, nephritis, pneumonia, arthritis

–> adult horse: pyosepticaemia

–> swine: septicaemia, arthritis

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16
Q

A. suis:

  • Habitat, staining, morphology, culture, pathogenicity
Study These Flashcards
A

Habitat:

  • swine mucous membranes (respiratory and genital tract)

Staining, morphology:

  • gram negative, middle sized rods

Culture:

  • blood agar, b-hemolysis

Pathogenicity:

  • Swine (grower, adult): pneumonia, septicaemia, metritis, abortion, arthritis
  • suckling piglet: septicaemia
17
Q

A. seminis

  • habitat, culture, pathogenicity
Study These Flashcards
A

Habitat:

  • mucous membrane of the genial tract of rams and bocks, semen

culture:

  • 10 % CO2 blood agar

Pathogenicity:

  • facultative pathogenic, inflammation of the accessory glands, epididymitis, abortion
18
Q

A. ureae

  • habitat, pathogenicity
Study These Flashcards
A

Habitat:

  • human respiratory way

Pathogenicity:

  • human upper respiratory infection
19
Q

A. pleuropneumoniae

  • Habitat, Morphology
Study These Flashcards
A

Habitat:

  • mucous membranes of the respiratory tract of swine

Morphology:

  • coocoid, short rods, some strains have fimbria
20
Q

A. pleuropneumoniae

  • culture
Study These Flashcards
A
  • fastidious
  • b-hemolysis
  • blood agar, chocolate agar with yeast extract
  • biotypes: NAD (V factor) demand

–> biotype 1: V-factor demand: satellitism, NAD, fresh yeast extract

–> Biotype 2: V-factor is not needed

21
Q

A. pleuropneumoniae

  • biochemistry, antigens, resistance, pathogenicity
Study These Flashcards
A

Biochemistry:

  • Apx I-II-III-IV cytotoxin produced

–> damage of macrophages and endothelial cells

Antigens:

  • 18 serogroups

Resistance: weak

Pathogenicity:

  • swine haemorrhagic-necrotic pleuropneumonia
22
Q

Haemophilus

  • habitat, morphology, staining
Study These Flashcards
A

Habitat:

  • mucous membranes of humans and animals (respiratory, genital)

Morphology:

  • 0.5-2 um coccoid rods, filaments, (fimbria)

Staining:

  • Gram negative
23
Q

Haemophilus

  • Culture
Study These Flashcards
A
  • fastidious
  • chocolate agar
  • additives:

–> haem (X-factor): blood

–> NAD (V-factor): satellitism, NAD, fresh yeast extract

  • some species need 10-15% CO2
24
Q

Haemophilus

  • biochemistry, antigens, resistance, pathogenicity
Study These Flashcards
A

Biochemistry:

  • examination is difficult

Antigens:

  • cell wall antigens
  • capsule antigens

Resistance: weak

Pathogenicity:

  • facultative pathogenic
25
Haemophilus - species
H. influenzae: - guman upper respiratory ways - meningitis H. parasuis: - V-factor demand - 15 serotypes - porcine polyserositis (Glasser´s disease)
26
Histophilus - species names, habitat, staining, morphology, culture
Species name?: - Histofilus somni (Haemophilus somnus, histophilus ovis, Haemophilus agni) Habitat: - urogenital mucous membranes, respiratory way Staining, morphology: - gram negative, short rods Culture: - fastidious - X, V factor not needed - CO2 demand
27
Histophilus - Pathogenicity
Facultative pathogenic Cattle: - calf pneumonia - septicaemia, - thromboembolic meningoencephalitis (TEME) - abortion, mastitis Sheep: - epididymitis-orchitis - abortion - mastitis - lamb septicaemia
28
Avibacterium - Habitat, morphology, staining, culture
Habitat: - respiratory mucous membrane Morphology: - 0.5-2 um coccoid rods, filaments Staining: - gram negative Culture: - fastidious - V-factor demand is variable - some species need CO2
29
Avibacterium - species
Avibacterium (Haemophilius) paragallinarum: - CO2 and V-factor are needed - 3 serogroups, 9 subgroups - infectious coryza of hens A. gallinarum - V-factor is not needed - mild respiratory disease in hens A. avium - V-factor is needed - saprophyte, sometimes mild respiratory disease in hens
30
Other genera having shared characteristics with the family Pasteurellaceae:
- Ornithobacterium - Rimerella - Taylorella
31
Ornithobacterium - habitat, morpholoy, staining
Ornithobacterium rhinotracheale Habitat: - some bird species (turkey, chicken) upper respiratory ways Morphology: - 1-3 um rods Staining: - Gram negative - bipolar
32
Ornithobacterium - culture, biochemistry, resistance, pathogenicity
Culture: - fastidious, blood agar - 10% CO2 Biochemistry: - catalase -, oxidase + Resistance: weak Pathogenicity: - facultative pathogenic - turkey, chicken bronchitis, upper respiratory infection
33
Riemerella - habitat, morphology, staining
Riemerella anatipestifer Habitat: - water fowl, upper respiratory ways Morphology: - 1-3 um rods Staining: - gram negative, bipolar
34
Riemerella - culture, biochemistry, resistance, pathogenicity
Culture: - fastidious - blood agar, chocolate agar - 10% CO2 Biochemistry: - extracellular enzymes - proteolytic Resistance: weak Pathogenicity: - facultative pathogenic - anatipestifer disease in water fowl
35
Taylorella - habitat, morphology, staining, culture, pathogenicity
Tayorella (Haemophilus) equigenitalis Habitat: - stallions: genital mucous membranes - clitoris of mares Morphology: - gram negatice coccoid rods, filaments Culture: - fastidious - X and V factor not needed, CO2-demand Pathogenicity: - CEM: contagious equine metritis
36
Identification of an unknown bacterium, steps:
1. inoculation of an appropriate media 2. selection of suspected colonies, production of pure cultures 3. examination of cultural characteristics and colony morphology 4. primary tests 4. secondary tests 5. identification of the bacterium 6. examination of the genome if necessary 7. examination of antibiotic resistance
37
Identification of an unknown bacterium culture - 4. primary and secondary tests
4. Primary tests: - Gram staining, spores - examination of movement - catalase production - oxidase production - oxidative - fermentative test (glucose, aerobic/anaerobic prop.) - identification of the genus or family 4. Secondary tests: - examination of enzymes and products of the carbohydrate metabolism - examination of enzymes and products of the nitrogen metabolism - detection of extra cellular enzymes

Bacteriology (10 decks)

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