Lab final

Question 1

What are some of the most common types of bacteria?

Answer 1

bacillus, spherical (cocci), helical or curved

Question 2

Answer 2

cococ bacilus

Question 3

Answer 3

rod (bacilus)

Question 4

Answer 4

staphylococci

Question 5

Answer 5

streptococci

Question 6

what are the common types of plating?

Answer 6

streak plate and pour plate

Question 7

what is the primary stain in gram staining?

Answer 7

crystal violet

Question 8

What is the mordant in graim staining?

Answer 8

iodine

Question 9

what is the decolorizer in gram staining?

Answer 9

alcohol

Question 10

what is the counter strain in gram staining?

Answer 10

safranin

Question 11

what causes the staining in gram staining?

Answer 11

gram positive has a thicker wall of peptiloglycan

Question 12

What result is this picture (gram stain)?

Answer 12

gram negative

Question 13

what result is this gram stain?

Answer 13

gram positive

Question 14

what species make endospores?

Answer 14

bacillus and clastridia

Question 15

what is exosporium?

Answer 15

protective coating of endospore

Question 16

where can endospores be made?

Answer 16

autoclave

Question 17

what is mycolic acid?

Answer 17

protective coating on some bacteria like leprosy and tuberculosis

Question 18

what is the primary stain in acid-fast?

Answer 18

carbolfuchsin

Question 19

what do cells that are acid fast look like?

Answer 19

dark red

Question 20

what colour is non-acid fast stain?

Answer 20

blue

Question 21

what is motility determined by?

Answer 21

flagella

Question 22

what is chemotaxis?

Answer 22

causes organism with flagella to move due to danger

Question 23

what kind of rings does gram positive flagella have?

Answer 23

S and M rings

Question 24

how can motility be determined?

Answer 24

by a wet mount test or hanging drop method

Question 25

what is a psycrophile?

Answer 25

growth between -5 and 20. supercool

Question 26

what is a mesophile?

Answer 26

optimal growth between 50 and 20

Question 27

what is a thermophile?

Answer 27

50 to 80

Question 28

what is a hyperhermophile?

Question 29

What is hypotonic?

Answer 29

outside is lower

Question 30

what is hypertonic?

Answer 30

greater outside

Question 31

what is a halotolerant organism?

Answer 31

tolerates salt like staph

Question 32

what is the difference between antimicrobials and antibiotics?

Answer 32

antibiotics kill or inhibit as well as antimicrobials

Question 33

Answer 33

filiform

Question 34

Answer 34

beaded

Question 35

Answer 35

echinulate

Question 36

Answer 36

effuse

Question 37

Answer 37

arborescent

Question 38

Answer 38

ring

Question 39

Answer 39

pellice

Question 40

Answer 40

flocculent

Question 41

Answer 41

f

Question 42

Question 43

what is a negative result of glucose?

Answer 43

green in both tubes

Question 44

what kind of result is this in glucose test?

Answer 44

fermentative and oxidative

Question 45

what is this result in a glucose test?

Answer 45

oxidative

Question 46

what results does this show in a durham sugar tube?

Answer 46

positive and negative

Question 47

what kind of results does this show for methyl red test?

Answer 47

positive and negative

Question 48

what is the VP test results shown?

Answer 48

positive and negative

Question 49

what does the methyl red test show?

Answer 49

shows mixed acid fermentation

Question 50

what does the durham sugar test show?

Answer 50

fermentation of sugars

Question 51

what does the VP test show?

Answer 51

ferment gucoe to make limited amounts of organic acids and butanadiol

Question 52

what does the citrate test show?

Answer 52

citrate as a carbon source

Question 53

is this a positive or nagtive citrate result?

Answer 53

positive blue

Question 54

what is the results seen for the oxidative test?

Answer 54

purpose positive

Question 55

what does the oxidative test show?

Answer 55

whether it can survive in an environment of oxygen

Question 56

what result is this in starch hydrolysis?

Answer 56

clear zone is positive for strach hydrolysis

Question 57

which is a positive result for casein hydrolysis?

Answer 57

clear zone on right indicates casein hydrolysis

Question 58

what does casein hydrolysis mean?

Answer 58

means that bacteria makes protealase

Question 59

which result if positive for fat hydrolysis?

Answer 59

dark blue pigmentation on left

Question 60

which of the following is positive tryptone hydrolysis?

Answer 60

the one wth the red ring at top

Question 61

what does tryptone degradation show?

Answer 61

that it degrades tryptophan to pyruvate

Question 62

what does a positive urease test look like?

Answer 62

pink tube

Question 63

what is the purpose of the urea test?

Answer 63

presence of urease

Question 64

what does kiglers iron agar do?

Answer 64

shows the fermenation of lactose and glucose

Question 65

what does this result show in kigler's iron agar?

Answer 65

alkaline no change

Question 66

what does this result in kigler's iron agar show?

Answer 66

hydrogren sulfide profuction

Question 67

what does this result in kigler's iron agar show?

Answer 67

acid/acid with gas

Question 68

what does this result for kigler's iron agar show?

Answer 68

alkaline and alkaline

Question 69

what does this result show in SIM medium?

Answer 69

hydrogen sulfide production

Question 70

what does this result show in SI medium?

Answer 70

indole production

Question 71

what are the results shown in this litmus milk test?

Answer 71

alkaline , acid, peptonization, coagulation

Question 72

what do the different litmus milk tests show?

Answer 72

alkaline- release of ammonia acid- ferments milk sugar peptonization- proteolytic that degrade protein

Question 73

what are the two types of hemolysis?

Answer 73

alpha and beta

Question 74

what does beta hemolysis look like?

Answer 74

clear zone with no red blood cells

Question 75

what color is a positive bile esculin test?

Answer 75

black