Lab Final Flashcards
(167 cards)
Breeding soundness exam purpose
- is to breed genetically superior, healthy males
- after BSE is complete the male is classified as satisfactory, questionable or unsatisfactory
Primary Follicles
- an oocyte surrounded by a single layer of cuboidal epithelial cells
- will either move forward with folliculogenesis or degenerate
Cattle estrous cycle length
- estrous cycle of a cow or heifer is about 21 days
- the estrus cycle lasts between 6-24 hours
Pars intermedia
- the dividing line between the two tissue types of the posterior and anterior pituitary glands
Vulva
- external portion of the female reproductive tract
- labia major and labia minor (meet to form two commissures, site of union)
- Perineum is located around the external genitalia
- clitoris
Alveoli
- are sack-like structures where milk is synthesized and secreted
- cluster of alveolus
Specificity
- does the assay detect one substance or cross-react and potentially detect others
2 Tissue Layers of the Ovary
- Germinal epithelium
- outer layer of the ovary - Tunica Albuginea
- underneath the germinal epithelium
Mare Estrus behaviors
- urination stance
- urination in the presence of a stallion
- clitoral wink
- immobile stance
- > teaser stallion to elicit response in mare
Ewe estrus behaviors
- nervous/excited behavior
- interest in the ram
- tail wagging
- > presence of a male is necessary to elicit behavior
- > presence of a ram can stimulate non-cycling ewes to ovulate 3-4 days after contact with the ram (RAM EFFECT)
Estrous
- adj. cycle beginning at estrus and ending at estrus
Intralobular Ducts
- drain multiple lobules within a single lobe
Germinal Disc
- the fertilized ova and will develop into the chick
- white spot on the yolk
Disadvantages of Ultrasound
- highly dependent on the skill of the technician to interpret what is being seen
- can lead to negative/false positives
Diffuse Placenta
- horses and pigs
Primordial Follicle in the ovary
- an oocyte surrounded by a single layer of squamous epithelium
- after puberty, the primordial follicles will develop into primary follicles
What is indicative of fertilization occurring
- white membrane forms around the oocyte (fertilization envelope)
- spinning oocyte
Why do we use ultrasound in animal agriculture today?
- to determine if an animal is pregnant
Oviduct/Fallopian Tube
- tubular structure made of 3 sections
1. Infundibulum
2. Ampulla
3. Isthmus
Neurohypophysis
- pars nervosa
- posterior pituitary
B-mode or real time ultrasound
- uses 2D real time imaging
- scanning tissue cross sections at different angles and reconstructing the data into a 3D image
Indirect ELISA
- wells are coated with antigens, a specific type of antibody
- Add the sample and allow antibodies to attach to antigen
- Add a secondary antibody that is tagged with an enzyme is added
- Add substrate to produce the color change
- Color change indicates presence of hormone
Cotyledon
- the fetal side of the placenta
What happens when a cow is milked?
- the sphincter muscles relax allowing the orifice to open
- since the streak canal remains open for an hour or more after milking there is ready access of bacteria to the inside of the gland
- during the dry period the epidermal tissue lining the streak canal forms a keratin plug that effectively seals off the canal