Lab Final Flashcards
(60 cards)
Logarithmic Graphs are used to…
the dependent variable plots as…
…when it is proportional to…
- represent large numbers in a short space
- a straight line
- a constant power of the
independent variable.
Semi-logarithmic Graphs are used when…
successive values of one quantity increase in geometric
progression as another quantity increases in arithmetic progression.
Linear Scale Graphs are used when…
relationships are in non-geometric progression.
The independent variable belongs on the
The dependent variable belongs on the
x-axis (horizontal line)
y-axis (vertical line)
What is bolded in a figure/graph
Table 1/Figure1
Where does the title go:
on a graph =
on a table =
graph = title below table = title below
What is the difference between a logarithmic and semi-logarithmic graph?
Log graphs have x & y axis as log functions (also used when we have large values in a small scale)
Semi-log graphs have either x or y as log functions (also used when one value is progressing geometrically but the other is progressing arithmetically)
True or False:
Dependent variables are plotted on the abscissa
False, the independent variable is plotted on the abscissa
x axis =
y axis =
x = abscissa = independent y = ordinate = dependent
practice quiz 1 question 3
plzzzz don’t u want a big fat throbbing AAAAA
practice quiz 1 question 4
plzzzz don’t u want a big fat wet AAAAA
What is calibration? Explain calibration relative to photospectrometry:
Calibration is comparing what you have to what is known in order to set a standard/control in an experiment without calibration in photospectrometry values would be inaccurate
True or False: Working distance (distance between the objective and the slide where the slide is in focus) of the microscope increases as the magnification power of the objective lens increases
False
The revolving power improves by changing the yellow light source to (red or violet)
violet
resolving power equation
d = lambda / (NAoc - NAobj)
Higher wavelength means _____ resolving power
lower
the resolution can improve with the increase or decrease in numerical aperture
increase
monochromatic
light of one wavelength/nm (incident beam Io)
True or False:
A standard curve can be established by measuring the absorbance of a solution at various concentrations
True
- Cell fractionation is…
- by either…
- …a general procedure that consists of disrupting the cell membrane (and cell wall of plants) to release the cell contents and then separate the organelles based upon differences in size or density
- …differential centrifugation or density gradient centrifugation.
isoelectric point (pI) =
the pH at which a particular molecule carries no net electrical charge or is electrically neutral
At a pH below their pI, proteins carry…
At a pH above their pI, proteins carry…
…a net positive charge
…a net negative charge.
- @ its pI, proteins are at their…
- Therefore, by changing the pH of the solution to the pI, the…
…lowest solubility (due to net 0 charge)
…solubility of the protein will be lowered
- As the salt concentration is increased…
- Further increases in the salt concentration result in…
- Finally, the protein starts to…
- This phenomenon of protein precipitation in the presence of excess salt is known as…
… a point of maximum protein solubility is usually reached
…fewer water molecules available to solubilize protein (since the water molecules are being used to solubilize the salts)
…precipitate when there is not a sufficient number of water molecules to interact with (solubilize) the protein molecules
…salting-out.