Flashcards in Quiz 3 Deck (8)
isoelectric point (pI) =
the pH at which a particular molecule carries no net electrical charge or is electrically neutral
At a pH below their pI, proteins carry...
At a pH above their pI, proteins carry...
...a net positive charge
...a net negative charge.
- @ its pI, proteins are at their...
- Therefore, by changing the pH of the solution to the pI, the...
...lowest solubility (due to net 0 charge)
...solubility of the protein will be lowered
Bradford Assay is based on...
When the dye binds to the protein...
The amount of light absorbance at a wavelength of 595 nm is directly proportional to...
- the simple binding affinity for a particular dye (Bio Rad Protein Assay Dye) to protein.
- it changes from red to blue.
- the amount of protein
= the utilization of various stains and chemical staining procedures to visualize where specific cellular components are located in tissue sections (or thin layers of cells)
The basic steps in histotechnique: (4)
1.) The tissue or organ is removed from the organism and preserved = FIXATION
2.) The fixed tissue is sectioned (thinly sliced) using a microtome = SECTIONING
3.) The sectioned tissue is placed on a microscope slide and stained = STAINING
4.) The stained tissue sections are then permanently mounted = MOUNTING
Difference between native and non-native gel electrophoresis:
Native electrophoresis the proteins are held in their normal (Native) form
whereas with non–native electrophoresis the proteins are denatured (i.e., their normal 3-D structure is disturbed).