[LAB] Permanent Stains

Question 1

[COMPONENTS]
IODINE

Answer 1

LUGOL’S SOLUTION
- 100mL Distilled Water
- 10g Potassium Iodide
- 5g Iodine Crystals

GRAM’S IODINE
- 1 part Lugol’s Solution
- 14 parts Distilled Water

Question 2

[COMPONENTS]
LUGOL’S SOLUTION

Answer 2

• 100mL Distilled Water
• 10g Potassium Iodide
• 5g Iodine Crystals

Question 3

[COMPONENTS]
GRAM’S IODINE

Answer 3

• 1 part Lugol’s Solution
• 14 parts Distilled Water

Question 4

[COMPONENTS]
MODIFIED D’ ANTONI’S IODINE

Answer 4

-100 mL Distilled Water
- 1g Potassium Iodide
- 1.5g Powdered Iodine Crystals

Question 5

STORAGE CONDITION OF MODIFIED D’ ANTONI’S IODINE

Answer 5

STORE IN BROWN BOTTLE, AWAY FROM LIGHT

Question 6

TIME WHEN D’ ANTONI’S IODINE CAN BE USED AFTER PREPARATION

Answer 6

4 DAYS

Question 7

[COMPONENTS]
MERTHIOLATE IODINE FORMALDEHYDE

Answer 7

• 0.10mL Lugol’s Solution
• 0.125mL Formaldehyde
• 0.774 mL Tincture of Merthiolate

Question 8

TECHNIQUE OF WHEATLEY

Answer 8

TRICHROME STAIN

Question 9

MODIFICATION OF GOMORI’S STAINING

Answer 9

TRICHROME STAIN

Question 10

[COMPONENTS]
TRICHROME STAIN

Answer 10

• 0.6g Chromotrope 2R
• 0.3g Light Green SF
• 0.7g Phosphotungstic Acid
• 1mL Glacial Acetic Acid
• 100mL Distilled Water

Question 11

[RESULTS]
TRICHROME STAIN
CYTOPLASM

Answer 11

BLUE-GREEN
WITH A TINGE OF PURPLE

Question 12

[RESULTS]
TRICHROME STAIN
NUCLEI AND INCLUSIONS

Answer 12

SLIGHTLY MORE PURPLE

Question 13

[RESULTS]
TRICHROME STAIN
BACKGROUND MATERIAL

Answer 13

GREEN

Question 14

[STAIN]
CYTOPLASM IS STAINED BLUE-GREEN WITH A TINGE OF PURPLE

Answer 14

TRICHROME STAIN

Question 15

[STAIN]
NUCLEI AND INCLUSIONS ARE COLORED RED

Answer 15

TRICHROME STAIN

Question 16

[STAIN]
CYST IS STAINED SLIGHTLY MORE PURPLE

Answer 16

TRICHROME STAIN

Question 17

[STAIN]
BACKGROUND AND MATERIALS ARE STAINED GREEN

Answer 17

TRICHROME STAIN

Question 18

[COMPONENTS]
IRON HEMATOXYLIN STAIN
MODIFIED SPENCER MONROE METHOD

Answer 18

STOCK SOLUTION
- 10g Hematoxylin Crystals
-100 mL 95% Ethanol

WORKING SOLUTION
- 1 part Hematoxylin Stock Solution
- 19 parts distilled Water

Question 19

OTHER TERM FOR THE IRON HEMATOXYLIN STAINC

Answer 19

MODIFIED SPENCER MONROE METHOD

Question 20

OTHER TERM FOR MODIFIED SPENCER MONROE METHOD

Answer 20

IRON HEMATOXYLIN STAIN

Question 21

[RESULTS]
IRON HEMATOXYLIN STAIN
BACKGROUND

Answer 21

BLUE GRAY

Question 22

[RESULTS]
IRON HEMATOXYLIN STAIN
CELLS AND ORGANISMS

Answer 22

VARIOUS INTENSITIES OF BLUE-GRAY

Question 23

[RESULTS]
IRON HEMATOXYLIN STAIN
INCLUSIONS, CHROMATOIDAL BODIES, AND NUCLEAR STRUCTURES

Answer 23

DARKER THAN THE SURROUNDING CYTOPLASM

Question 24

[STAIN]
BACKGROUND MATERIAL IS STAINED BLUE-GRAY

Answer 24

IRON HEMATOXYLIN STAIN
MODIFIED SPENCER-MONROE METHOD

Question 25

[STAIN] CELLS AND ORGANISMS ARE STAINED VARIOUS INTENSITIES OF BLUE GRAY

Answer 25

IRON HEMATOXYLIN STAIN MODIFIED SPENCER-MONROE METHOD

Question 26

[STAIN] INCLUSIONS, CHROMATOIDAL BODIES, AND NUCLEAR STRUCTURES ARE STAINED DARKER THAN THE SURROUNDING CYTOPLASM

Answer 26

IRON HEMATOXYLIN STAIN MODIFIED SPENCER-MONROE METHOD

Question 27

WHAT DOES CALCOFLUOR WHITE STAIN

Answer 27

ACANTHAMOEBA CYSTS

Question 28

[COMPONENTS] CALCOFLUOR WHITE

Answer 28

DISODIUM SALT OF 4,4-BIS-(4-ANILINO-BIS-DIETHYLAMINO-5-TRIAZIN-2-YLAMINO)-2,2-ATILBENE DISULFONIC ACID

Question 29

NON SPECIFIC FLUOROSCENT DYE

Answer 29

CALCOFLUOR WHITE

Question 30

[STAINS] ACANTHAMOEBA CYSTS

Answer 30

CALCOFLUOR WHITE

Question 31

[RESULTS] ACANTHAMOEBA CYST STAINED WITH CALCOFLUOR WHITE

Answer 31

DOUBLE WALLED OUTER WALL IS WRINKLED CYCTS WILL FLUORESCE

Question 32

NEGATIVE CONTROL OF CALCOFLUOR WHITE

Answer 32

E.coli WILL NOT FLUORESCE MOST BACTERIA AND OTHER DEBRIS WILL NOT FLUORESCE SOME YEAST AND DEBRIS MAY

Question 33

SPECIAL STAINS FOR MICROSPORIDIA

Answer 33

MODIFIED TRICHROME-WEBER GREEN MODIFIED TRICHROME-RYAN BLUE

Question 34

[STAINS] PENETRATES THE SPORES OF THE PARASITES

Answer 34

MODIFIED TRICHROME-WEBER GREEN MODIFIED TRICHROME-RYAN BLUE

Question 35

SPECIAL STAINS FOR COCCIDIA

Answer 35

MODIFIED KINYOUN’S ACID-FAST STAIN (COLD) MODIFIED ZIEHL-NEELSEN ACID-FAST STAIN (HOT)

Question 36

[STAIN] COLD METHOD

Answer 36

MODIFIED KINYOUN’S ACID FAST STAIN

Question 37

[STAIN] HOT METHOD

Answer 37

MODIFIED ZIEHL-NEELSEN ACID FAST STAIN

Question 38

[COMPONENTS] KINYOUN’S CARBOL FUCHSIN

Answer 38

- 4g Basic Fuchsin - 8g Liquified Phenol - 20mL 95% Ethanol - 100mL Distilled Water

Question 39

[COMPONENTS] LOEFFLER’S ALKALINE METHYLENE BLUE

Answer 39

- 0.3g Methylene Blue - 30mL 95% Ethanol - 100mL KOH - Sulfuric Acid (Decolorizer)