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PARASITOLOGY > [LAB] Stool Concentration Techniques > Flashcards

[LAB] Stool Concentration Techniques Flashcards

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1
Q

PROCEDURE THAT ALLOWS THE DETECTION OF PARASITES THAT HAVE BEEN MISSED BY THE DFS

A

STOOL CONCENTRATION TECHNIQUE

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2
Q

PARASITES THAT CAN BE RECOVERED WITH THE STOOL CONCENTRATIOIN TECHNIQUE

A

HELMINTH EGGS AND LARVAE
PROTOZOAN CYSTS

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3
Q

PARASITES THAT CANNOT BE RECOVERED BY THE STOOL CONCENTRATION TECHNIQUE

A

PROTOZOAN TROPHOZOITES
They are usually destroyed during the procedure

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4
Q

TWO CONCENTRATION TECHNIQUES

A

SEDIMENTATION
Formalin Ether Concentration Technique

FLOTATION
Zinc Sulfate Concentration Technique

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5
Q

[FECT]
VOLUME AND CONCENTRATION OF FORMALIN

A

10 mL
10% Formalin

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6
Q

[FECT]
AMOUNT OF FECES

A

1.0-1.5g
HALF A TEASPOON

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7
Q

MATERIAL USED TO STRAIN THE SOLUTIONS

A

GAUZE
2 LAYERS

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8
Q

[FECT]
TOTAL VOLUME

A

10 mL

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9
Q

[FECT]
VOLUME OF ETHER OR ETHYL ACETATE

A

3mL

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10
Q

[FECT]
DURATION OF SHAKING

A

10 SECONDS

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11
Q

[FECT]
RATIONALE OF REMOVING THE RUBBER STOPPER AFTER SHAKING

A

RELEASE PENT-UP AEROSOL OF ETHER

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12
Q

[FECT]
DURATION OF CENTRIFUGATION

A

10 MINUTES

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13
Q

[FECT]
NUMBER OF LAYERS

A

4 LAYERS

  1. Ether or Ethyl Acetate
  2. Fatty debris
  3. Formalin
  4. Sediment
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14
Q

[FECT]
4 LAYERS AFTER CENTRIFUGATION

A

4 LAYERS

  1. Ether or Ethyl Acetate
  2. Fatty debris
  3. Formalin
  4. Sediment
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15
Q

[FECT]
LAYERS TO POUR OFF

A

TOP 3 LAYERS

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16
Q

[FECT]
DURATION OF DRAINING THE SOLUTION

A

AT LEAST 5 SECONDS

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17
Q

[FECT]
WHAT TO DO WITH THE RESIDUAL FLUID FROM THE WALLS OF THE TUBE

A

MIX THE FLUID WITH THE SEDIMENT

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18
Q

[FECT]
AMOUNT OF THE SEDIMENT NEEDED ON THE GLASS SLIDE

A

A DROP

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19
Q

[FECT]
STAIN USED

A

IODINE

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20
Q

WHY SHOULD ETHER BE HANDLED WITH CARE

A

ETHER IS HIGHLY FLAMMABLE AND WILL IGNITE IF A SPARK IS NEAR BY

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21
Q

WHAT SUBSTANCE IS HIGHLY FLAMMABLE AND WILL IGNITE IF A FLAME IS NEARBY

A

ETHER

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22
Q

[ZINC SULFATE]
HOW TO PREPARE A FECAL EMULSION

A

FECAL SPECIMEN
About half the size of a thumb

WATER

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23
Q

[ZINC SULFATE]
VOLUME OF SUSPENSION

A

10 mL

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24
Q

[ZINC SULFATE]
CENTRIFUGATION SPEED AND DURATION

A

MANUAL: 2000 RPM, 12 MINUTES
SUPP. NOTES: 500g, 10 MINUTES

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25
[ZINC SULFATE] SUBSTANCE USED TO WASH THE FECAL SEDIMENT
WATER
26
[ZINC SULFATE] UNTIL WHEN SHOULD THE SUSPENSION BE WASHED AND CENTRIFUGED
UNTIL THE SUPERNATANT IS CLEAR
27
[ZINC SULFATE] AMOUNT OF WASHINGS USUALLY DONE
3 WASHINGS
28
[ZINC SULFATE] WHAT TO DO WITH THE LAST SUPERNATANT FLUID
POUR IT OUT
29
[ZINC SULFATE] VOLUME AND CONCENTRATION OF ZINC SULFATE USED
2 mL 33% ZINC SULFATE
30
[ZINC SULFATE] SPECIFIC GRAVITY OF FRESH STOOL
1.18
31
[ZINC SULFATE] SPECIFIC GRAVITY OF FORMALIN-PRESERVED STOOL
1.20
32
[ZINC SULFATE] AMOUNT OF LOOPS TRANSFERRED TO THE GLASS SLIDE
5 LOOPFULS OF THE TOP PORTION
33
TECHNIQUE THAT SUSPENDS THE FECAL SPECIMEN IN WATER OR AQUEOUS SOLUTION OF LOW SPECIFIC GRAVITY
SEDIMENTATION Formalin Ether Concentration Technique
34
[SEDIMENTATION OR FLOTATION] SOLUTION OF LOW SPECIFIC GRAVITY
SEDIMENTATION Formalin Ether Concentration Technique
35
[SEDIMENTATION OR FLOTATION] NATURAL SETTING
SEDIMENTATION Formalin Ether Concentration Technique
36
[SEDIMENTATION OR FLOTATION] PARASITES AT THE BOTTOM OF THE SUSPENSION
SEDIMENTATION Formalin Ether Concentration Technique
37
[SEDIMENTATION OR FLOTATION] DEBRIS IS LIGHT
SEDIMENTATION Formalin Ether Concentration Technique
38
[SEDIMENTATION OR FLOTATION] HIGH SPECIFIC GRAVITY
FLOTATION Zinc Sulfate Concentration Technique
39
[SEDIMENTATION OR FLOTATION] PARASITES ARE SEPARATED ACCORDING TO BUOYANCY
FLOTATION Zinc Sulfate Concentration Technique
40
[SEDIMENTATION OR FLOTATION] PARASITES ARE LOCATED IN THE SURFACE FILM
FLOTATION Zinc Sulfate Concentration Technique
41
ALTERNATIVE TO ETHYL ACETATE
GASOLINE
42
MOST WIDELY USED SEDIMENTATION TECHNIQUE
FECT
43
PROCEDURE USED TO SCREEN FOOD HANDLERS AND OFWs
FECT
44
RECOMMENDED SEDIMENTATION PROCEDURE BY THE DEPARTMENT OF HEALTH
FECT
45
PRINCIPLE IS BASED ON SPECIFIC GRAVITY
FECT ans ZSCT ?
46
FUNCTION OF ETHER
EXTRACTOR OF DEBRIS AND FAT FROM FECES
47
SUBSTANCE USED TO EXTRACT DEBRIS AND FAT FROM FECES
ETHER
48
WIDELY RECOMMENDED SUBSTITURE FOR ETHER
ETHYL ACETATE
49
ETHYL ACETATE CAN BE A SUBSTITUTE FOR WHAT SUBSTANCE
ETHER
50
SUBSTANCE USED FOR THE SAFE RECOVERY OF G. lamblia CYSTS Taenia spp. H. nana eggs
ETHYL ACETATE
51
ETHYL ACETATE IS A SAFER EXTRACTOR FOR THE RECOVERY OF WHAT PARASITES
G. lamblia CYSTS Taenia spp. H. nana eggs
52
[FECT] AMOUNT OF TIME NEEDED EBFORE THE STOPPER IS REMOVED
15 TO 30 SECONDS
53
[FECT] CENTRIFUGATION SPEED AND DURATION
(CURRENT RECOMMENDATION) 500g MINIMUM OF 10 MINUTES
54
[FECT] PARASITES THAT MAY NOT BE RECOVERED IF CENTRIFUGATION TIME IS LOWERED
COCCIDIA MICROSPORIDIA
55
[FECT] IS TAPPING OF THE TUBE ALLOWED
NO IT WILL DISTURB THE SEDIMENT
56
[FECT] EFFECT OF EXCESS ETHYL ACETATE IN THE SMEAR OF THE SEDIMENT
BUBBLE FORMATION
57
[FECT] CAUSE OF BUBBLE FORMATION
EXCESS ETHYL ACETATE IN THE SMEAR OF THE SEDIMENT
58
[FECT] USE OF IODINE
STAIN ENHANCES MORPHOLOGICAL DETAIL
59
[FECT] ADVANTAGES
RECOVERS A BROAD RANGE OF ORGANISMS LEAST SUBJECT TO TECHNICAL ERROR CAN STORE SEDIMENTS FOR LONG PERIODS OF TIME EXTRACTS AND DISSOLVES FATS OR MUCOIDAL MATERIALS
60
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] EASY TO PERFORM
ADVANTAGE FECT
61
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] ALLOWS RECOVERY OF THE BROADEST RANGE OF ORGANISMS
ADVANTAGE FECT
62
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] LEAST SUBJECT TO TECHNICAL ERROR
ADVANTAGE FECT
63
DISADVANTAGES OF FECT
USE OF ETHER CONTAINS MORE DEBRIS NO PROTOZOAN TROPHOZOITES
64
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] USE OF ETHER
DISADVANTAGE FECT
65
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] CONTAINS MORE DEBRIS
DISADVANTAGE FECT
66
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] NO PROTOZOAN TROPHOZOITES
DISADVANTAGE FECT
67
ADVANTAGES OF ZINC SULFATE TECHNIQUE
YIELDS A CLEAR PREPARATION
68
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] YIELDS A CLEAR PREPARATION
ADVANTAGE ZINC SULFATE
69
DISADVANTAGES OF THE ZINC SULFATE TECHNIQUE
PROTOZOAN CYSTS AND THIN SHELLED HELMINTH EGGS ARE PRONE TO COLLAPSING AND DISTORTION SOME HELMINTH EGGS DO NOT CONCENTRATE WELL CANNOT BE USED FOR FATTY STOOL OR TOO HARD STOOL
70
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] PROTOZOAN CYSTS AND THIN SHELLED HELMINTH EGGS ARE SUBJECT TO COLLAPSE AND DISTORTION
DISADVANTAGE ZINC SULFATE
71
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] SOME HELMINTH EGGS DO NOT CONCENTRATE WELL
DISADVANTAGE ZINC SULFATE ASCARIS EGGS
72
[ADVANTAGE OR DISADVANTAGE] [FECT OR ZINC SULFATE] CANNOT BE USED FOR FATTY STOOL OR TOO HARD STOOL
DISADVANTAGE ZINC SULFATE
73
WHAT HAPPENS TO OPERCULATED EGGS IN THE ZINC SULFATE TECHNIQUE
HIGH SPECIFIC GRAVITY OF ZINC SULFATE CAUSES THE OPERCULA TO POP EGGS FILL WITH FLUID EGGS SINK TO THE BOTTOM RATHER THAN FLOAT TO THE TOP (SURFACE FILM)
74
WHAT HAPPENS TO UNFERTILIZED A. lumbricoides EGGS IN THE ZINC SULFATE TECHNIQUE
VERY DENSE SINK TO THE BOTTOM OF THE SOLUTION
75
WHEN ARE STOOL CONCENTRATION TECHNIQUES DONE
IF DFS (-) BUT S/S(+) OF AN INFECTION
76
[ZINC SULFATE] AMOUNT OF STOOL USED
HALF A TEASPOON OF FRESH STOOL
77
[ZINC SULFATE] VOLUME OF WATER USED
ABOUT 10 mL
78
[ZINC SULFATE] PARASITE THAT RUPTURES WHEN DISTILLED WATER IS ADDED
Blastocystis hominis
79
[ZINC SULFATE] ALTERNATIVE SOLUTION FOR DISTILLED WATER
0.85% NaCl 5-10% FORMALIN
80
[ZINC SULFATE] STANDING TIME FOR MIXTURE FIXATION
30 MINUTES
81
[ZINC SULFATE] THREE (TWO) LAYERS
1. Surface Film 2. Zinc Sulfate 3. Sediment (Debris)
82
[ZINC SULFATE] AMOUNT OF DROPS OF THE SURFACE FILM USED
2 DROPS
83
[ZINC SULFATE] ALTERNATIVE INOCULATING MATERIAL USED INSTEAD OF THE LOOP
PASTEUR PIPETTE
84
[ZINC SULFATE] TIME LIMIT FOR REMOVING THE SURFACE FILM AFTER CENTRIFUGATION
WITHIN 5 MINUTES
85
[ZINC SULFATE] EFFECT OF PROLONGED SUSPENSION IN ZINC SULFATE ON PARASITES
COLLAPSE AND DISTORTION
86
[TRUE OR FALSE] IF ZINC SULFATE IS THE ONLY METHOD USED, BOTH THE SURFACE FILM AND THE SEDIMENT CAN BE EXAMINED
TRUE
87
PURPOSE OF STOOL CONCENTRATION TECHNIQUE
AGGREGATE PARASITES PRESENT IN A SMALL VOLUME REMOVE DEBRIS

PARASITOLOGY (5 decks)

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