Lab Practicum

Question 1

Glucose A/A LIA P/P

Indole + Citrate =

Urea =

Answer 1

E. coli

Question 2

Causes pneumonia and meningitis

Answer 2

Hemophilus influenzae

Question 2

Gram + pleomorphic rods in parallel lines, Ks, and Vs

Orange growth on chocolate

Normal skin flora

Answer 2

Corynebacterium sp or diptheroids

Question 2

Gram = coccobacillary; TSI K/K; Motility=; OF Glucose+; Maltose=; Oxidase =

Answer 2

Acinetobacter

Question 3

If you have suspected S. aureus from yellow MSA plate, what test should you do to confirm?

Answer 3

Coag test should be performed, clot confirms S. aureus

Question 4

Phenylalanine + genera

Answer 4

Proteus

Providencia

Morganella

Question 5

Sputum smear shows Gram + lancet shaped diplococci

A disc +

P disc +

Answer 5

Streptococcus pneumoniae

Question 6

A disc contains bacitracin and contains zone for

Answer 6

Group A Beta Strep

Question 8

Grey and mucoid

Growth only around XV on Mueller Hinton plate

Tiny Gram = pleomorphic rods

Answer 8

Hemophilus influenzae

Question 8

Dry pink, >100 colonies on MacConkey indicates

Answer 8

UTI

Question 9

SBA showing streaks for isolation but only little dot growth in one line

Answer 9

Hemophilus influenzae, called the satellite phenomenon

Question 10

Camp test +

Answer 10

Group B Beta Streptococcus

Question 10

What test should be done first if you see Gram + cocci

Answer 10

catalase test

Question 11

Causes pneumonia, TSS, boils carbuncles

Answer 11

Staphylococcus aureus

Question 11

Yellow growth on XLD (red)

Answer 11

Lactose fermenter

Question 12

Yellow growth on MSA

Answer 12

Staphylococcus aureus

Question 14

Mucoid growth; A/A; Citrate+; Motility=; Ornithine=; Indole=

Answer 14

Klebsiella pneumoniae

Question 16

Swarming growth on chocolate

Swarming growth on SBA

CNA =

Clear growth on MacConkey

Indole =

Answer 16

Proteus mirabilis

Question 17

Mucoid growth; A/A; Citrate +; Motility =; Ornithine =; Indole +

Answer 17

Klebsiella oxytoca

Question 18

If you have a wound culture with growth on SBA, chocolate, and a pink colony on MacConkey, what should you identify

Answer 18

the pink colony

Question 19

EMB with green metallic sheen

Answer 19

E. coli

Question 20

P/P on LIA means

Answer 20

lysine +

Question 21

Swarming growth on SBA

Swarming growth on chocolate

CNA =

Clear growth on MacConkey

Indole +

Answer 21

Proteus vulgaris

Question 22

TSI A/A; Whitish gray with very narrow zone of β hemolysis; BEA + (black); Motility + (umbrella); Gram + rods

Answer 22

Listeria

Question 24

\>100,000 Gray, moist, non-hemolytic on SBA MacConkey = BEA + (black) NaCl +

Answer 24

Group D Enterococcus

Question 25

Clear growth on MacConkey; Oxidase+; GNF red; 42P+; Blue/metallic; Most commonly isolated nonfermentor

Answer 25

Pseudomonas aeruginosa

Question 26

Neutrophils with Gram = coffee bean shaped diplococci intracellularly; Gray colones on Chocolate and Thayer Martin plates Dextrose + Lactose = Maltose = Sucrose=

Answer 26

Neisseria gonnorhaea

Question 27

Clotted Coag Hemolysis on SBA Cream colored colonies on Chocolate agar Hemolysis on CNA MacConkey = Gram + cocci clusters

Answer 27

Staphylococcus aureus

Question 29

Gram + Spores Hemolytic on SBA

Answer 29

Bacillus

Question 30

TSI K/A with H₂S (red/yellow)

Answer 30

Salmonella

Question 31

GNF tube yellow at butt

Answer 31

ferments glucose

Question 32

Orange growth on HE (green)

Answer 32

Lactose fermentor