Lab Quiz 2

Question 1

What is the NMB staining procedure

Answer 1

3 drops of NMB
2 drops of blood
Mix well and have for 15 minutes
Incubate for 10 mins at 37C
Make 3 smears
Let it dry

Question 2

What is not visible in a Wright stain

Answer 2

Reticulocytes
Heinz bodies
Hemoglobin H

Question 3

Answer 3

Polychromasia

Question 4

Answer 4

Basophilic stippling

Question 5

Answer 5

Supravital stains - reticulocyte

Question 6

A reticulocyte stain measures

Answer 6

Aggregates of residual ribosomes and mitochondria that form clumped granular material called reticulum

Question 7

% retics =

Answer 7

(Number of retics/1000)*100

Question 8

For anemia, retic index (corrected) % =

Answer 8

(% retics* hematocrit of patient)/ normal hematocrit (45%)

Question 9

For young reticulocytes, reticulocyte production index (RPI) =

Answer 9

Retic index/maturation time in days

Question 10

Answer 10

Blister cell

Question 11

Answer 11

Bite cell

Question 12

Answer 12

Heinz bodies

Question 13

How are Heinz bodies formed

Answer 13

By damage to the hemoglobin component molecules, usually through oxidative damage by administered drugs, or from an inherited mutation

Question 14

What are denatured Hgb due to oxidative stress

Answer 14

Heinz bodies

Question 15

Answer 15

Hemoglobin H disease
Note: tear drops and blister cells

Question 16

Answer 16

Supravital stain - hemoglobin H
Lots of Heinz bodies

Question 17

Hemoglobin H is made up of

Answer 17

4 beta sheets

Question 18

Study differences

Answer 18

Question 19

Answer 19

Pappenheimar bodies
(Abnormal granules of iron)

Question 20

What stain is Perl’s reaction

Answer 20

Prussian blue stain

Question 21

What does the Prussian blue stain (perl’s reaction) demonstrate

Answer 21

Iron stores in bone marrow or sideroblasts on peripheral blood smear

Question 22

Answer 22

Positive iron stain in bone marrow

Question 23

Answer 23

Negative iron stain in bone marrow

Question 24

Answer 24

Howell-jolly bodies
(Nuclear remnants) fragments of DNA

Question 25

Answer 25

Cabot rings (Remnants from a mitotic spindle)

Question 26

Answer 26

Rouleaux "Roll of pennies”

Question 27

Makes cells less neg. Allowing them to touch

Answer 27

High protein gathering electrons

Question 28

Answer 28

RBC agglutination

Question 29

Name and survival rate

Answer 29

Neutrophils 1-2 days

Question 30

Answer 30

Band neutrophils

Question 31

Answer 31

Toxic granulation

Question 32

Answer 32

Dohle bodies

Question 33

Answer 33

Vacuoles

Question 34

Answer 34

Blue-green inclusions “Death crystals”

Question 35

Name the disease and morphology

Answer 35

Iron deficiency anemia Microcytic hypochromic

Question 36

Name the disease

Answer 36

Megaloblastic anemia Macrocytic anemia

Question 37

Name the disease and characteristics

Answer 37

Hereditary spherocytosis Spherocytes-variable, polychromasia BM pushing more cells out

Question 38

Name the disease and characteristics

Answer 38

Hereditary elliptocytosis Elliptocytes, mild anemias

Question 39

Answer 39

Eosinophil

Question 40

Answer 40

From left to right: Segmented neutrophil Basophil Monocyte

Question 41

Answer 41

Basophil

Question 42

Answer 42

Monocytes

Question 43

Answer 43

Lymphocytes

Question 44

Name the disease and characteristics

Answer 44

Iron deficiency anemia Microcytes, hypochromasia, often high platelets

Question 45

Name the disease and characteristics

Answer 45

Severe iron deficiency Microcytic, hypochromic, elliptocytes, tear drops, bizarre forms, often high platelet count

Question 46

Name the disease and characteristics

Answer 46

Sideroblastic anemia Can have pappenheimer bodies, one population hypochromic microcytic the other normal

Question 47

Name the cell

Answer 47

Ringed sideroblasts (bone marrow)

Question 48

Name the disease and characteristics/morphology

Answer 48

Megaloblastic anemia Oval macrocytes, howell-jolly bodies, tear drop (dacryocytes), hyper-segmented neutrophil, Cabot ring

Question 49

Does the white count, red count, and platelets increase or decrease in megaloblastic anemia

Answer 49

Decrease

Question 50

Name the disease and characteristics

Answer 50

Pernicious anemia Oval monocytes, tear drops, hyper-segmented neutrophils

Question 51

Name the disease and characteristics

Answer 51

Liver disease Macrocytes, Target cells, acanthocytes High/increased MCV

Question 52

Name the disease and characteristics

Answer 52

Hereditary pyropoikliocytosis Elliptocytes/ovalocytes, red cell fragments, severe microcytosis

Question 53

Name the disease and characteristics

Answer 53

Thermal burns Spherocytes, fragmented red cells, microvesicles, no Polychromasia, rapidly cleared

Question 54

Answer 54

Immune hemolytic anemia

Question 55

Name the disease and characteristics

Answer 55

Hereditary spherocytosis (post splenectomy) Spherocytosis, polychromasia

Question 56

Name the disease and characteristics

Answer 56

Microangiopathic anemia Schistocytes, little to no platelets, sepsis, inappropriate clotting

Question 57

Name the disease and characteristics

Answer 57

Marked eosinophilia Drug allergies

Question 58

Name the cell and characteristics

Answer 58

Basophil Water soluble granules can dissolve during staining No pink granules (neutrophil)

Question 59

Name the disease and its characteristics

Answer 59

Sepsis Toxic neutrophils, toxic granulation, vacuolization

Question 60

Name the disease and its characteristics

Answer 60

Monocytosis Irregular shaped nuclei, light areas Tuberculosis, listeriosis, malignancy