Lab Quiz 3 Flashcards
(34 cards)
What are neutrophils, eosinophils, basophils, lymphocytes, and monocytes?
They are all white blood cells
Which of the following is not a granulocyte? Check all that apply.
monocyte
lymphocyte
The largest cells in the blood that leave the bloodstream to become macrophages are the:
monocytes
Match the following leukocytes with their relative abundance.
Neutrophils 55-56%
Eosinophils 1-3%
Basophils 0.2%
Lymphocytes 25-33%
Monocytes 3-7%
Match the following WBCs with their function.
phyagocytosis, mostly bacteria neutrophils
attack parasites eosinophils
sustain inflammatory response by releasing heparin and histamine basophils
active in specific acquired immunity Lymphocytes
phagocytosis and antigen presentation monocytes
PMNs are another name for:
neutrophils
Mature neutrophils are also called segs because the nucleus is segmented into 2-5 lobes Immature neutrophils are also called Immature neutrophils are also called bands because the nucleus lacks segmentation.
Mature neutrophils are also called segs because the nucleus is segmented into 2-5 lobes Immature neutrophils are also called Immature neutrophils are also called bands because the nucleus lacks segmentation.
Hematopoiesis occurs in which of the following?
bone marrow
Match the following UV radiation to their corresponding wavelengths.
UV-A 315-400 nm
UV-B 280-315 nm
UV-C 100-280 nm
Rank the following microorganisms from the least resistant (1) to the most resistant (4):
1 G+ bacteria
2 G- bacteria
3 mycobacteria
4 endospores
Which bacteria do we use for the UV radiation experiment? Check all that apply.
B. subtilis
E. coli
Which bacterium do you expect to be more resistant to UV radiation?
B. subtilis because it produces endospores
The short wavelength UV is able to penetrate through the plastic cover of petri dish.
False
Explain the mechanism of action of UV radiation on cells.
UV causes damage to DNA by causing formation of pyrimidine dimers.
Which bacteria do we use for the chemical germicide experiment? Check all that apply.
E. coli
S. aureus
P. aeruginosa
Which of the following are disinfectants that we will test? Check all that apply.
Lysol
Bleach
Which of the following are antiseptics that we will test? Check all that apply.
Isopropyl alcohol
Hydrogen peroxide
Arrange the chemical germicide experiment steps in the correct order.
1 coat beads with bacteria
2 dry beads on filter paper
3 incubate beads in germicides
4 place beads into nutrient broth
Match the following antibiotics with their mode of action.
Cell wall synthesis and function, vancomycin
50S ribosome inhibition, chloramphenicol
30S ribosome inhibition, tetracyclines
cell membrane permeability, polymyxins
Folic acid synthesis, trimethoprim
DNA/RNA synthesis, rifampin
You have just measured the zone of inhibition for several drugs against Staphylococcus. It just happens that all of them are about 14 mm. What does this mean for each drug?
(Use the ‘Zone diameter interpretive chart’ on page 449 of your lab manual, and the ‘Kirby Bauer antibiotic sensitivityLinks to an external site.’ sheet posted on Canvas as resources).
Chloramphenicol, intermediate
Penicillin, resistant
Polymyxin, sensitive
These factors determine the size of a zone of inhibition. Mark all that apply.
the thickness of the agar medium
Rate of diffusion
Drug solubility
Choose one antibiotic that you would expect to show different effects on Gram-positive and Gram-negative bacteria. Which bacterium is more susceptible? Why do you think this might be the case?
Polymyxin B, Gram positive should be more susceptible since it does not have an outer membrane making it easier for membrane disruption to occur
Explain the difference between facultative anaerobes and aerotolerant microbes. Which group has more extensive bioenergetic enzyme system? Explain.
Both can use oxygen, but facultative anaerobes much prefer to use oxygen and are able to produce more energy when they do have access to oxygen.
Facultative anaerobes since they can work with both the present and absence of oxygen.
What are the purposes of the indicator strip and the gas generator in the GasPak system in the anaerobic jar?
creates an anaerobic chamber for organisms, the strip is used to indicate the oxygen presence
