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Lab Quiz 3 Flashcards

1
Q

What is the purpose of the decarboxylase test?

5-11: Decarboxylase

A

To determine whether or not bacteria can decarboxylate lysine or ornithine

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2
Q

What is the name of the medium?

5-11: Decarboxylase

A

Lysine/ornothine broth
- LDC or ODC

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3
Q

What is the substrate, bacterial enzyme, and end product?

5-11: Decarboxylase

A

Substrate: Lysine or Ornothine
Bacterial Enzyme: Lysine or Ornothine Decarboxylase (endoenzyme)
End Products: Cataverine or putrescine (alkaline diamines) + CO2 g

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4
Q

What is the appearance of a positive/negative?

5-11: Decarboxylase

A

Positive: Purple/gray
Negative: Yellow

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5
Q

What is the reagent and pH indicator?

5-11: Decarboxylase

A
  • None
  • Bromocresol purple
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6
Q

What else is important about this test? (4)

5-11: Decarboxylase

A
  • Results cannot be read before 48H
  • Anaerobic enzymes
  • Decarboxylation is anaerobic (oil used)
  • Glucose is used first then acids which activate decarboxylase enzymes
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7
Q

What is the purpose of the deaminase test?

5-12: Deaminase

A

To determine whether bacteria can deaminate phenylalanine

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8
Q

What is the name of the medium?

5-12: Deaminase

A

Phenylalanine agar

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9
Q

What is the substrate, bacterial enzyme, and end product?

5-12: Deaminase

A

Substrate: Phenylalanine
Bacterial enzyme: Phenolalanine deaminase (endoenzyme)
End product: Ammonia + phenylpyruvic acid

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10
Q

What is the appearance of a positive/negative?

5-12: Deaminase

A

Positive: Green
Negative: Yellow

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11
Q

What is the reagent and pH indicator?

5-12: Deaminase

A

Reagent: Ferric Chloride (FeCl3) (indicates phenols)
pH indicator: None

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12
Q

Is protein deamination and decarboxylation an anaerobic or aerobic process?

A

Deamination - aerobic
Decarboxylation - anaerobic

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13
Q

What else is important about this test?(2)

5-12: Deaminase

A
  • pH products neutralize eachother
  • Aerobic enzyme
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14
Q

What is the purpose of the casease test?

5-16: Casease

A

To determine whether bacteria can hydrolyze casein

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15
Q

What is the name of the medium?

5-16: Casease

A

Milk agar

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16
Q

What is the substrate, bacterial enzyme and end products?

5-16: Casease

A

Substrate: Casein
Bacterial enzyme: Casease (exoenzyme)
End products: AA + Polypeptides

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17
Q

What is a positive/negative result?

5-16: Casease

A

Positive: Clear zone
Negative: White

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18
Q

Are there reagents or pH indicators?

5-16: Casease

A

None

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19
Q

What else is important about this test?(1)

5-16: Casease

A
  • Both + and - organisms still grow
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20
Q

What is the purpose of the urease test?

5-18 Urease

A

To determine if bacteria can hydrolyze urea

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21
Q

What is the name of the medium?

5-18 Urease

A

Urea tablet

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22
Q

What is the substrate, bacterial enzyme, and end products?

5-18 Urease

A

Substrate: Urea
Bacterial enzyme: Urease
End products: Ammonia + CO2 g

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23
Q

What is a positive/negative result?

5-18 Urease

A

Positive: Pink
Negative: Yellow

24
Q

What are the reagents an pH indicators?

5-18 Urease

A

None
Phenol Red

25
What else is important about this test?(2) ## Footnote 5-18 Urease
- Ammonia burns mucous membranes and neutralizes stomach acid - *H. pylori*
26
What is the purpose of the TSI agar test? ## Footnote 5-21: TSI Agar H2S test
To differentiate bacteria on the basis of glucose fermentation, lactose fermentation, sucrose fermentation, and sulfur reduction
27
What is the name of the medium? ## Footnote 5-21: TSI Agar H2S test
TSI agar
28
What are the substrates, bacterial enzymes, and end products? ## Footnote 5-21: TSI Agar H2S test
Substrates: Cystine + Sodium thiosulfate Bacterial enzyme: Cystine desulfurase + Thiosulfate reductase (endoenzymes) End product: H2S
29
What is a positive/negative result? ## Footnote 5-21: TSI Agar H2S test
Positive: Any black Negative: No Black
30
What is the reagent and H2S indicator? ## Footnote 5-21: TSI Agar H2S test
No reagent Ferrous ammonium sulfate
31
What else is important to consider?(1) ## Footnote 5-21: TSI Agar H2S test
Black color indicates an acidic pH
32
What is the name of the medium? ## Footnote 5-21: TSI Agar pH test
TSI agar
33
What are the substrates, bacterial enzymes, and end products? ## Footnote 5-21: TSI Agar pH test
Substrates: Glucose, Lactose, Sucrose Bacterial enzymes: Various endoenzymes End products: Acids
34
What does a positive/negative result look like? ## Footnote 5-21: TSI Agar pH test
Positive: Some yellow (glucose only) Positive: All yellow (glucose plus at least one dissacharide) Negative: No yellow or black
35
What are the reagents and pH indicator used? ## Footnote 5-21: TSI Agar pH test
None Phenol Red
36
What else is important about this test?(1) ## Footnote 5-21: TSI Agar pH test
- In glucose only ferm. (K/A slant) glucose was in low % and runs out, peptones are then used aerobically
37
What are the results of this test? ## Footnote 5-21: TSI Agar HS2 and pH
- K/A H2S- - A/A H2S- - K/K H2S- - K/K H2S- - A/A H2S+ - K/A H2S+
38
What is the purpose of the oxidase test? ## Footnote 5-7: Oxidase
To test bacteria for oxidase enzyme
39
What is the name of the medium? ## Footnote 5-7: Oxidase
Nutrient agar
40
What is the substrate, bacterial enzyme, and end product? ## Footnote 5-7: Oxidase
Substrate: O2 Bacterial enzyme: Cytochrome C oxidase (endoenzyme) End product: H2O
41
What is a positive/negative result? ## Footnote 5-7: Oxidase
Positive: Red/pink Negative: No change
42
What is the reagent and pH indicator? ## Footnote 5-7: Oxidase
Oxidase reagent None
43
What is the purpose of the catalase test? ## Footnote 5-6: Catalase
To test bacteria for the catalase enzyme
44
What is the name of the medium? ## Footnote 5-6: Catalase
Nutrient agar
45
What is the substrate, bacterial enzyme, and end products? ## Footnote 5-6: Catalase
Substrate: H2O2 Bacterial enzyme: Catalase (endoenzyme) End products: H2O + O2 g
46
What is a positive/ negative result? ## Footnote 5-6: Catalase
Positive: Bubbling Negative: No bubbles
47
What reagent and pH indicator were used? ## Footnote 5-6: Catalase
H2O2 None
48
What is the purpose of the anaerobic jar test? ## Footnote 2-8: Anaerobic Jar
To determine the oxygen requirements of an organism
49
What is the name of the medium? ## Footnote 2-8: Anaerobic Jar
Nutrient agar
50
What is the substrate, chemical, and end product? ## Footnote 2-8: Anaerobic Jar
Substrate: O2 Chemical: Anaerogen chemical End product: H2O
51
What is a positive/negative result? ## Footnote 2-8: Anaerobic Jar
Positive: Growth Negative: No growth
52
What is the purpose of the thiobroth test? ## Footnote 2-7: Thiobroth
To determine the O2 requirements of an organism
53
What is the name of the medium? ## Footnote 2-7: Thiobroth
Thio broth
54
What is the purpose of the sodium thioglycollate and L-cystine in the recipe? ## Footnote 2-7: Thiobroth
Remove O2 to make anaerobic environment (turned to water)
55
What do these results mean? ## Footnote 2-7: Thiobroth
- Growth only at top means the organism is a obligate aerobe - Growth at top and in stab means the organism is a facultative anaerobe - Growth only in the stab means obligate anaerobe - Thin growth near the top means organism is a microaerophile
56
What enzyme is present in aerobic bacteria? Anaerobic? ## Footnote 2-7: Thiobroth
Superoxide dismutase - aerobic Catalase - anaerobic

Microbiology 210 (13 decks)

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