Lab Quiz 3 (Lab 5 & 6) Flashcards by Kaleb Mehaffey

The ____ test tests for the enzyme catalase

Drop ____ on the bacteria to perform the test

Bubbles = Positive

This test cannot be performed on bacteria on blood agar because blood contains catalease and a false positive may occur

Catalease

3% hydrogen peroxide

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The top catalase test is a ____

Positive

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The ____ tests for the conversion of nitrate or to ammonia or nitrogen

Nitrate

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The Nitrate test uses ____ as its medium

The Nitrate test uses ____ and ____ as its reagents

Nitrate broth

Nitrate Reagent A & Nitrate Reagent B

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In the Nitrate test, you first:

Add 5 drops of ____ and ____
Look for red color
If no red color, add ____
Look for red color

Nitrate Reagent A & Nitrate Reagent B
Zinc dust

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In the Nitrate test, red color after the addition of nitrate reagents indicates the bacteria have changed the nitrate to ____

No color change after the addition of zinc indidates the bacteria have converted nitrates to ____ or nitrogen gas

nitrite

ammonia

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The Mannitol Salt Agar (MSA) is selective for ____ such as Staphylococcus

Halophiles

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With the Mannitol Salt Agar (MSA) test, you must report two results: ____ & ____

Growth and Fermentation

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With the Mannitol Salt Agar (MSA) test, you streak for ____

Isolation

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This MSA test reports

No Growth (NG)

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This MSA test reports

Growth with No Fermentation (G-)

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This MSA test reports

Growth with Fermentation (G+)

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The ____ Test uses Bacitracin, which is a peptide antibiotic that inhibits cell wall synthesis in a susceptible bacteria

Bacitracin Sensitivity

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The Bacitracin Sensitivity uses an ____ plate as the medium

SBA

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In the Bacitracin Sensitivity Test, sensitive bacteria will have a ____, resistent bacteria will not

zone of inhibition

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According to the Bacitracin Sensitivity Test, this bacteria is:

Sensitive

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According to the Bacitracin Sensitivity Test, this bacteria is:

Resistent

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In the Novobiocin test, sensitive bacteria will have a ____. Resistent bacteria will not.

Zone of Inhibition

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In the Novobiocin test, you swap an ____ plate and place down novobiocin disks using sterible forceps

SBA

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In the Novobiocin test on the left is

Resistent

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In the Novobiocin test on the right is

Sensitive

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The Coagulase test on the top is ____

Positive

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The Coagulase test on the bottom is ____

Negative

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The ____ test tests for the production of the enzyme coagulase

It is also used to differentate between staphylococcus species

Coagulase

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To perform a Coagulase test, you first add bacteria to coagulase ____ (from rabbits) Incubate Look for coagulation of the plasma

plasma

A positive result in the Coagulase test is \_\_\_\_

Coagulation

With the Hemolysis on Sheep's Blood Agar Test, you first: Streak for \_\_\_\_ Incubate Observe hemolysis by holding the plate up to a light

isolation

\_\_\_\_ hemolysis is complete hemolysis and will result in a clear zone around the colonies

Beta

This is ____ hemolysis

Beta

\_\_\_\_ hemolysis is partial hemolysis and will result in a greenish zone around the colonies

Alpha

This is ____ hemolysis

Alpha

\_\_\_\_ hemolysis is no hemolysis and is indicated by no zone around the colonies

Gamma

This is ____ hemolysis

Gamma

The Voges-Prokauer Test tests for the production of a ____ end product from fermentation The Voges-Prokauer Test uses ____ as a medium

neutral MR-VP Broth

**Steps of the Voges-Prokauer Test** 1. Innoculate ____ broth. Incubate for 48 hours 2. Add \_\_\_\_, \_\_\_\_, and \_\_\_\_ 3. Incubate at room temperature and look for a ____ color

1. MR-VP 2. VP Reagent A, VP Reagent B, Creatine 3. Red

The VP Test on the left is \_\_\_\_

positive

The VP Test on the right is \_\_\_\_

negative

The Growth in 6.5% NaCl broth test is selective for \_\_\_\_

halophiles

**NaCl broth test** The tube on the left is \_\_\_\_

Positive

Growth in 6.5% NaCl broth test A positive will have growth in the tube, which will appear as \_\_\_\_

turbidity

In the Bible Esculin test, ____ is your medium

Bile Esculin agar slant

the Bible Esculin test inhibits the growth of other ____ organisms

Gram +

Bible Esculin Test The tube in the middle is \_\_\_\_

Negative

Bible Esculin Test The tube on the right is \_\_\_\_

Positive

With the Bile Esulin test, hydrolysis of esculin causes a black ____ and should be reported as + (black) or - (not black)

precipitate

The ____ Test tests for the production of amalyase in bacteria

Starch Hydrolysis

Starch Hydrolysis Test 1. Inoculate the organism by streaking a single line down the center of a ____ plate 2. Incubate at 37°C 3. Flood the plate with \_\_\_\_ 4. Let the plate sit for a couple of minutes and pour the solution down the sink 5. Observe the plate for a ____ around the bacteria

1. Starch Agar 3. Iodine 5. Clear zone

Starch Hydrolysis Test Iodine reacts with starch in the plate and turns it a dark purple/black color. A clear zone around the bacteria indicates the search has been ____ and is a ____ result

Hydrolyzed; Positive

The ____ Test is useful in identifying Streptococcus pneumonia. The growth of S. pneumonia is inhibited by it.

Optochin Sensitivity

The Optochin Sensistivity Test steps: 1. Swab an ____ plate with bacteria in three directions 2. Using sterile forceps, place a ____ on the plate

1. SBA 2. optichin disk

The Sulfur Indole Mobility Test (SIM) can be used to operform three separate tests in one tube. 1. Tests for the production of \_\_\_\_ 2. Conversion of tryptophan to \_\_\_\_ 3. Ability to move through semi-solid agar due to the presence of ____ (mobility test)

1. H₂S 2. indole 3. flagella

Steps of the Sulfur Indole Mobility Test (SIM) 1. Stab a ____ deep 2. Incubate **For mobility test**: Look for growth of bacteria at sites distant from the initial point of inoculation **For H₂S production**: Look for a ____ precipitate **For Indole test**: Add a layer of ____ reagent and look for a ____ color

1. SIM **For mobility test**: Look for growth of bacteria at sites distant from the initial point of inoculation **For H₂S production**: Look for a ***black*** precipitate **For Indole test**: Add a layer of ***Kovac's*** reagent and look for a ***red*** color

Sulfur Indole Mobility Test (SIM) **Mobility potion** The tube on the left is \_\_\_\_ The tube in the middle is \_\_\_\_ The tube on the right is \_\_\_\_

Negative Positive Positive

Sulfur Indole Mobility Test (SIM) **Indole potion** The tube on the left is \_\_\_\_ The tube on the right is \_\_\_\_

Negative Positive

Sulfur Indole Mobility Test (SIM) **H₂S Production potion** The tube on the left is \_\_\_\_ The tube on the right is \_\_\_\_

Negative Positive

The Mannose Fermentation tests detects mannose using \_\_\_\_, which contains phenol red as an indicator

Cystine Tryptic Agar supplemented with mannose (CTA-M)

The Mannose Fermentation tests detects mannose using Cystine Tryptic Agar supplemented with mannose (CTA-M), which contains ____ as an indicator

phenol red

**Steps of the Mannose Fermentation test** 1. Inoculate ____ by stabbing with a inoculating needle 2. Incubate 3. Look for a ____ color and for \_\_\_\_, \_\_\_\_

1. CTA-M 3. yellow; cracks; bubbles

\_\_\_\_ Test tests for the hydrolysis of casein

The Casein Agar (Skim Milk Agar)

**Steps for The Casein Agar (Skim Milk Agar) Test** 1. Streak a single line down the center of the \_\_\_\_ 2. Incubate 3. Observe plates for a ____ around the line of bacteria

1. Casein Agar plate 3. Clear zone

**The Casein Agae (Skim Milk Agar) Test** The line on the left is a \_\_\_\_ The line on the right is a \_\_\_\_

Negative Positive

The ____ tests for the production of acidic end products from fermentation

Methyl Red Test

**Steps of the Methyl Red Test** 1. Inoculate \_\_\_\_ 2. Incubate 3. Add \_\_\_\_ 4. Mix 5. Look for ____ color

1. MR-VP broth 3. Methyl Red 5. Red

**The Methyl Red Test** The tube on the left is \_\_\_\_ The tube on the right is \_\_\_\_

Negative Positive

OPNG Test Some bacteria produce an enzyme, \_\_\_\_, which can hydrolyze OPNG into galactose and ortho-nitrophenol. The ortho-nitrophenol has a ____ color.

β-galatosidase; yellow

**Steps of the ONPG test** 1. Inoculate ____ using a heavy inoculum 2. Using sterile forceps, place a disk of ____ into the tube 3. Incubate for 24 hours 4. Look for a ____ color

1. ONPG broth 2. ONPG reagent 3. yellow

**OPNG test** The tube on the left is \_\_\_\_ The tube on the right is \_\_\_\_

Negative Positive

Lab Quiz 3 (Lab 5 & 6) Flashcards

(67 cards)