lab safety Flashcards

(60 cards)

1
Q

most danger route and uderestimated of exposure

A

inhalation of aerosoles

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2
Q

how many risk groups are there

A

4

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3
Q

most common risk contamination level in reserach lebs

A

2

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4
Q

what are aersoles

A

suspensions of solid or liquid particles that can enter resp

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5
Q

5 factors that determine infection due to aerosoels

A

viability of biohazard
conctration of aeorosoles
size
presisitance
susceptibility of host

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6
Q

3 ways to decontaminate lab waste

A

autoclave
disinfect
incinerate

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7
Q

temp of autoclave

A

121

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8
Q

most common disinfect for non met

A

belach, sodium hypochlorite

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9
Q

equipment that is used when working with agents that make aerosols

A

biosafety cabinets

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10
Q

test microgorganizm in auticlaves

A

geobacillus stearothermophilus

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11
Q

hepa stands for

A

high efficiency particulate air

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12
Q

standarsd for hepa filters are based on

A

removal of particles of 0.3 um

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13
Q

hepa is made from

A

densely filter of glass and paper fibers on aluminum or wood base

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14
Q

safety cabinets are tested whe 3

A

annualy, initially, moves/repairs

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15
Q

class of biological safety cabinets is based on 3

A

protection
air circulation
closed system and hepa filter type

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16
Q

class 1, 2 a1, a2, b1 issues

A

recirculation of air , toxins / flamable inot cabinet

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17
Q

leacking spciments clean up 7 steps

A

inform others
PPE
place speciment onto tray and move to cabinet
clean contamianted surface
in cabinet transfers contents to clean container
if requisutions is contaminated move to plastic bag
flood new tray and disinfect/ biohaz

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18
Q

non fixed slides are dangerous ?

A

yes

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19
Q

sds stands for

A

safety data sheets

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20
Q

5 things mandatory on sds

A

identification
handling
first aid
toxicolofy
physical and chemical properties

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21
Q

flammable limits

A

the range at which fuel will burn if ignitions source is there

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22
Q

LC50

A

concentration of substance in air will kill 50% animals

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23
Q

LD50

A

quantity of a substance death to 50% animals

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24
Q

TWA

A

concentration in air that will not harm if exposed to for 8 hrs

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25
STEL
15 min, for up to 4 times a day with no adverse affects
26
ceiling limit
should never be exceeded, toxic
27
flashpoint
concentration vaopur and soruce of ignition
28
4 types of aeorosoles
mists dusts smoke fumes
29
human carcinogen in lab
benzene
30
2 chmicals suspected of cancer
ethylene oxide formaldehyde
31
flammable
burns at room temp
32
combustible
must be heated before burn
33
oxidizing
material can provide oxygen to burn
34
corrosive
damage body tisssue
35
3 points to store oxidizers
away from flamables closed lids compatibility
36
does corrosive material just dosent effect pyrex glass
yes
37
are acids and basses corossive
yes
38
are reactive chemicals just alwyas dangerous
yes
39
danger chem in dry state
picric acid
40
laser means
light amplification by stimulated emission of radiation
41
is there no danger with laser
false there is
42
class A fire extin
paper, tectiles, plastics
43
class B fire
Oils
44
class C fire
electiric
45
class D fire
patassium metals
46
cryogenics fluids and ex
gasses that have been liquefied at low temp nitrogen
47
3 equipment when working with liquid nitrogen
full face mask imprevious clothing water proof gloves
48
egronomics keeps who in mind
worker
49
egronomics does not supoert 1
bending head
50
should wrists be raised up when typing
neur
51
breaks every 3/4 hrs for 2-3 min ?
neur, every hr
52
3 egroniics at computer
use wirist rest when not typing kevel monitor key board and mouse adjustable height
53
how long bf opening centrifuge
30 min
54
substance that cause birth defect
teratogenic
55
causes embryo harm
embryogenics
56
DNA harm
mutagenics
57
harm to fetus
fetagenic
58
w/ isolation patietns use
mask
59
when handling materials use
gloves
60
when workking with splatter use
apron