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Proc 180 > Mid 1 > Flashcards

Mid 1 Flashcards

(135 cards)

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1
Q

5 common glassware

A

High thermal resistant
High silica
High alkali resistant
Low actinic
standard flint

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2
Q

high thermal glass type

A

borosilicate

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3
Q

high thermal used for

A

beakers, flasks, pipettes

Resists heat changes

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4
Q

silica used for 3

A

optics, mirrors, cuvettes, thermometers

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5
Q

alkali glass, and used for

A

aluminosilicate
screen protectors- scratch resistants, alkaline resistant / corrosion

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6
Q

standard flint glass, and used for

A

soda lime
cheap: glass ware single use and dishes

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7
Q

5 plastic wares

A

polyolefins, polycarbonate, tygon (polyvinyl chloride), teflon, polystyrene

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8
Q

polyolefins 2 subs

A

polyethylene
polypropylene

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9
Q

polyolefins characteristics and used for

A

-unaffected by strong chemicals
-disposable: urine tubes, sample tubes, pipettes, bottles
-some autoclaves

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10
Q

polycarbonate characteristics and used for 2

A

-strong by susceptible to chemicals
-autoclavable
-centrifuge tubes and graduated cylinders
-not disposable

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11
Q

tygon 3 charac/uses

A

-non toxic
-flexible
-used in tubing
-clear
-handles most chemicals

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12
Q

teflon charac/purpose

A

-tough resin
-non-wettable/ is repelelnt
-stirring bars, stopcock, caps, tubing
-chemic innert

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13
Q

polystyrene charac/purpose

A

-chemic inert
-transparent
-not autoclavable
-microliter plates, petri dishes

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14
Q

most accurate flasks are

A

volumetric

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15
Q

graduated cylinders

A

less accurate but verstitile

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16
Q

beakers

A

not calibrated,
used as holding containers

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17
Q

Erlenmeyer flask

A

not calibrated
used for mixing to eliminate vapour

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18
Q

funnels

A

support filter

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19
Q

cuvettes

A

used in light measurement

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20
Q

fritted glass funnels

A

made from fine glass and different porosity used with filter paper, easy clean

-filters strong acids and bases

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21
Q

separating funnels

A

-separate immiscible liquids
-drop by drop filtering

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22
Q

test tubes can be made from

A

glass or plastic

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23
Q

centrifuge tubes

A

used to separate solids and liquids
plastic or though glass

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24
Q

3 types of cuvettes/ material

A

-disposable polystyrene
-quartz
-silica

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25
storage bottles characteristics
cylindrical narrow necks ground glass stoppers or screw tops
26
burets used for
titration or standardization procedure drop by drop
27
desiccators used for
drying out reagents and solids
28
2 types of desiccants and color
drierite barium oxide blue is dry pink is wet
29
non volumetric vs volumetric
not used for accuracy (just to aproximate) vs is
30
what class should all volumetric ware be in
class A analytic quality
31
To contain vs to deliver
to contain holds some liquid at the end that must be blown out, to deliver if drained properly does not need to be blown out
32
temp calibration for ware
20 C
33
fourth part on the glass ware is
units, ml ususally
34
volumetric glassware 3 ex
volumetric flasks, graduated cylinders pipette
35
3 steps to clean glass
-group -hot water -brush
36
new glasses are what and must be what
-alkaline -soaked in acid water -and rinsed
37
contaminated glass clean with
-10-20% bleach
38
last rinse should always be with
-distilled water
39
cloudy, dirty, coagulated organic matter cleaned with
chromatic acid
40
protein cleaned with
ammonium hydroxide
41
grease cleaned with
sodium carbonate
42
heavy metals cleaned with
dilute nitric acid
43
capacity of pipetted
1 uL to 100 mL
44
semi-automated capacity
100 uL-1.0 mL
45
macro capacity
> 0.1 mL
46
micro capcacity
<0.1 mL (100 uL)
47
concave meniscus for ... vs convex
clear opaque
48
mohr vs sero vs volumt TC or TD
mohr TD sero TC volum TD
49
tolerance
what liquid is actually being delivers %/100 x pipette mL = x minus pipette mL
50
tissue that wipes
Kimwipe
51
wipe semi auto only when and after always
working with blood rinse out
52
6 thermal equipment Hot and cold
refrigerators freezers water bath heating block air incubators hot air oven
53
refrigerators -temp -used for 3
- 4+- 2 -short term samples -blood for transfusions -reagents
54
freezers temp and use for 4
-20 or lower -long term plasma/serum -reagents -blood -cultures
55
water baths temp and in water reagents and dis/adv
37-56 -fungicide: thymol/thimersol -adv: quick heat and slow cooling -dis: the water in bath takes time to warm up and and its big
56
heating blocks temp and adv and dis
37-56 -adv: nothing gets wet -dis : less efficient heat transfer
57
air incubators temp , 2 types, usesful bc
37-56 -water jacket -anhydric -holds larger volume
58
hot air over temp used for
160 drying glass ware
59
microwaves
heat quickly and evenely
60
continous temp logs for 4
Everything / thermometers in all incubators, fridge, freezer
61
centirfuges
seperate materials based on densitiy rotation aroun central axis
62
resisitance will what to speed
decrease it
63
trunnion rings
hold buckets
64
buckets
hold test tubes
65
cushions
bottom oof bucket to prevnt breackage
66
two broad categ of cenrifuges
fixed angle and horizontal head
67
horizontal head 2 dis
greater resistance= heat, some remixing since we go from straight to side to straight
68
fixed angle effcient bc
45 degreee -less resistnace -can operate at higher speed
69
8 types of centrifugess
clinical floor model haematocrit ultra mega serofuge cell washer refrigerated
70
clinical -character -voulume
-small -routine -15-30 mL
71
floor model
-large volumes -many tubes -high speed/ better
72
haematocrit for and force
-small tubes -red cells bottom -14,000
73
ultracentirfuge speed and for
-60,000 -special urine -seperates densities
74
megafuge -use and spee
-large range of motors -routine -100-6000 rpm
75
serofuge
-serology -fixed angle -fast to reach speed
76
cell washer
-washes RDB with saline -decant to supernantant
77
refrigerated temp used
-15-25 -high speed but specimens not damaged from heat
78
how should u increase speed
gradually
79
clean centrifuge by
-bowl, head, accessories -dry cloth -mild detergent -drying oven -routine disinfection
80
how long bf open cent whne break
30 min
81
disinfect using what for cent
non-bleach
82
mechanical balance
relies on removable weight compensation load
83
electronic balance
electric current current is proportional to weight
84
vernier scale is used for capacity in g
weights on some mech balances microscopes 0.01 g
85
analytical balance fine vs micro vs moderate vs rough
most accurate measurments 0.0001 0.000001g 0.005-0.1 1
86
PH meters measure
H ion acitivty
87
2 types of electrodes and diff and made of
reference -constant voltage does not respond - silver chloride indicator - glass responds to H ions
88
combination elctrode
both in one body
89
2 sources of elctorode error
1- temp 2- reactive glass responds to more than H
90
electrode care
soack in neutral budder dont let glass dry out clean and gentle
91
LO1
92
is 0 odd or even
even
93
the round rule for 5 and 50
even always wins odd needs help
94
when multiplying integenrs
add exponents
95
when dividing integers
subtract exponents what you have - what you want
96
accuracy def -certainty -efficiency -truthfulness
correctness/ clossness to true value
97
precisions
reproducibility
98
between 1.3 and 1.27489 which is more precise
larger number
99
SI length mass volume time amount electric current thermodynamic temp
m g L s mol A K
100
in lab instead of K we use
C
101
numeric date order
year-month-day
102
time always has how many digits
4
103
prefixes order 13
Tera Giga Mega Kilo hecto deca deci centi milli micro nano pico fecto
104
hecto
2
105
deci
-1
106
deca
1
107
centi
-2
108
tera
12
109
mega
6
110
giga
9
111
kilo
3
112
milli
-3
113
nano
-9
114
micro
-6
115
pico
-12
116
fecto
-15
117
the numerical value should be between what
0.1 and 1000
118
should there be space btw unit and number
yes
119
solution
solute dissolved in a liquid solvent
120
aqueous vs non-aqueous
solvent is water vs solvent alcohol/ organic liquid
121
solute
substance dissolved in solvent to make solution
122
solvent
the liquid
123
dilute solutions
a small proportion of solute in solution ex 0.85%
124
concentrated solutions
large prop of solute ex 20%
125
saturated solutions
as much solute as solvent can dissolve ex 40%
126
supersaturate solutions
contains more solute than the solvent can keep dissolved
127
diluent
liquid into another liquid, usually the smaller one makes something fluid
128
dilution
taking a concentrated solution and adding diluent to it to make it less concentration
129
dilutions are statements of relative
concentrations not volume
130
ratio and proportions vs dilutions
the ratio is a 1 to x 1:9 and proportion would be 2:18 dilution is a 1 in x 1:10
131
concentration def
how much solute in a given volume
132
%w/v vs %v/v concentrations
g/100 mL mL/ 100mL
133
the weight of a substance is
the sum of the atomic weight of all atoms
134
hygroscopic or deliquescent are when how to dry
anhydrous substances absorb moisture from air and not 100% pure -drying oven or desiccator
135
hydrated chemicals are
substances crystalize in a way to unite with water and form hydrates

Proc 180 (4 decks)

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