[LAB] Specimen Collection

Question 1

Enumerate the order of draw.

Answer 1

Yellow - sterile
Light blue - sodium citrate
Red - no additives
Green - heparin
Lavender - EDTA
Gray - sodium fluoride or sodium oxalate

Question 2

What is the usual gauge size of a needle when collecting blood from an adult?

Answer 2

21-23

Question 3

What is the usual length of a needle when collecting blood using a syringe?

Answer 3

1-1.5in

Question 4

What is the method of collection when collecting blood from pediatric or geriatric populations?

Answer 4

Winged blood collection
“butterfly”

Question 5

What is the usual length of a needle when collecting blood using winged blood collection?

Answer 5

0.5-0.75in

Question 6

What is the length range of the plastic tubing used in the butterfly collection?

Answer 6

5-12in

Question 7

What is the result of a prolonged tourniquet application?

Answer 7

hemolysis and hemoconcentration

Question 8

What is the minimum time of tourniquet application before reapplying it?

Answer 8

2mins

Question 9

During the collection of blood, what is the maximum time for tourniquet application to avoid hemoconcentration and hemolysis?

Answer 9

1min

Question 10

What vein has a high likelihood of hematoma formation?

Answer 10

Basilic vein

Question 11

What vein is located on the thumb side of the arm?

Answer 11

Cephalic vein

Question 12

What is the vein of choice in venipuncture?

Answer 12

Median Cubital Vein

Question 13

What refers to the preferred site for venipuncture?

Answer 13

Antecubital fossa

Question 14

What is the appropriate way of anchoring the vein?

Answer 14

L-holding technique

Question 15

What is the angle of the needle in venipuncture?

Answer 15

15-30 degree angle

Question 16

What is the recommended method of blood collection from young children?

Answer 16

Capillary puncture

Question 17

Area for a capillary puncture on infants.

Answer 17

Lateral surface of the plantar side of the heel

Question 18

Area for a capillary puncture on children who started walking.

Answer 18

Plantar surface of big toe

Question 19

Area for a capillary puncture in adults.

Answer 19

The palmar surface of the distal or end segment of the middle or ring finger of non-dominant hand

Question 20

How deep is the puncture during capillary puncture?

Answer 20

less than 2mm

Question 21

Sites with cyanotic appearance indicates what?

Answer 21

decrease blood flow

Question 22

Enumerate the features of a well-made peripheral blood smear.

Answer 22

1. 2/3 to 3/4 length of the slide
2. finger-shaped film
3. visible lateral edges
4. smooth film
5. rainbow appearance in the feather edge

Question 23

What is the stain used in staining the blood smears?

Answer 23

Wright-giemsa

Question 24

Enumerate the components of Wright-Giemsa.

Answer 24

1. Methanol
2. Methylene blue
3. Eosin
4. Sodium phosphate or aged distilled water

Question 25

What is the pH of sodium phosphate?

Answer 25

6.4

Question 26

What is the pH range of aged distilled water?

Answer 26

6.4-6.8

Question 27

What refers to the technique when examining the peripheral blood smear?

Answer 27

battlement pattern

Question 28

What is the color of the peripheral blood smear macroscopically?

Answer 28

pink to purple

Question 29

At what part of the smear will you look when observing the cell morphology under OIO?

Answer 29

feather edge of the slide

Question 30

What is the color of the erythrocyte under OIO?

Answer 30

orange to salmon pink

Question 31

What is the time interval wherein will you check for a blood clot in the clotting time test?

Answer 31

every 30 seconds

Question 32

What is the normal reference range for clotting time procedure?

Answer 32

8-15 minutes

Question 33

What is the normal reference range for a bleeding time procedures?

Answer 33

2-4 minutes

Question 34

What is the depth of puncture when doing bleeding time procedure?

Answer 34

3mm

Question 35

What is the time interval wherein will you blot the filter paper with blood for a bleeding time procedure?

Answer 35

every 30 seconds

Question 36

What is the size of the blood drop in the slide when doing blood smear preparation?

Answer 36

1-2mm diameter

Question 37

What is the angle of the spreader slide when doing blood smear preparation?

Answer 37

30-40 degree

Question 38

What will you do when you put too much blood for the blood smear?

Answer 38

Raise the angle of spreader

Question 39

What will you do when you put too little blood for the blood smear?

Answer 39

Lower the angle of the spreader

Question 40

What will you do during blood smear preparation when the patient has a low hematocrit level?

Answer 40

Raise the angle of the spreader