Laboratory Equipment and Supplies

Question 1

tolerates heating and sterilization for lengthy periods; subject to scratching and may cloud with strong alkali

Answer 1

borosilicate (pyrex, kimax)

Question 2

6x stronger than borosilicate; better able to resist scratching and alkali

Answer 2

aluminosilicate (corex)

Question 3

heat, chemical, and electrical tolerance and excellent optical properties; used for high thermal, drastic shock, and extreme chemical treatment with acids and alkali

Answer 3

high silica (vycor)

Question 4

boron-free glass; used with strong acids and alkali

Answer 4

soft glass

Question 5

soda lime glass; most inexpensive; releases alkali causing errors in certain pH determinations

Answer 5

flint glass

Question 6

reduces light transmission

Answer 6

low actinic glass (amber-colored glass)

Question 7

borosilicate, aluminosilicate, high silica

a. ↑ thermal resistance
b. ↓ thermal resistance

Answer 7

a. ↑ thermal resistance

Question 8

soft glass, flint glass

a. ↑ thermal resistance
b. ↓ thermal resistance

Answer 8

b. ↓ thermal resistance

Question 9

excellent temp tolerance and chemical resistance

Answer 9

teflon

Question 10

used for pipet tips, test tubes, for cryogenic procedures (↓ temp.)

Answer 10

polypropylene

Question 11

stronger than polypropylene; used for centrifuge tubes, graduated cylinders

Answer 11

polycarbonate

Question 12

used for disposable transfer pipets, test tubes, bottles (binds/absorbs dyes, proteins, picric acids)

Answer 12

polyethylene

Question 13

rigid, clear; used for test tubes, graduated tubes

Answer 13

polystyrene

Question 14

soft, flexible but porous; frequently used as tubing

Answer 14

polyvinyl chloride

Question 15

teflon, polypropylene, polycarbonate

a. autoclavable, ↑ thermal resistance
b. non-autoclavable, ↓ thermal resistance

Answer 15

a. autoclavable, ↑ thermal resistance

Question 16

polyethylene, polystyrene, polyvinyl chloride

a. autoclavable, ↑ thermal resistance
b. non-autoclavable, ↓ thermal resistance

Answer 16

b. non-autoclavable, ↓ thermal resistance

Question 17

glass pipettes:

HOLDS but does not deliver the exact volume

Answer 17

to contain

Question 18

glass pipettes:

DELIVERS the exact volume it holds

Answer 18

to deliver

Question 19

glass pipettes:

etched ring/band near the mouth

Answer 19

blow-out pipet

Question 20

allowed to drain by GRAVITY

Answer 20

self-draining pipet

Question 21

example of blow-out pipettes with etched ring band

Answer 21

Ostwald-Folin
Serologic

Question 22

example of self-draining pipettes

Answer 22

Volumetric
Mohr

Question 23

Volumetric, Ostwald-Folin: what type?

a. transfer pipettes
b. measuring/graduated pipettes

Answer 23

a. transfer pipettes

Question 24

Serologic, Mohr: what type?

a. transfer pipettes
b. measuring/graduated pipettes

Answer 24

b. measuring/graduated pipettes

Question 25

graduated to tip

Answer 25

Serologic (si SERologic ay GRUMADUATE)

Question 26

graduated between 2 marks/ no graduation to tip; point to point delivery

Answer 26

Mohr (di pa grumaduate, kaya MOHR aral)

Question 27

Semi-automatic micropipettors: uses SUCTION to draw sample into a disposable polypropylene tip; piston does not come in contact with liquid

Answer 27

air displacement

Question 28

Semi-automatic micropipettors: - operates like a hypodermic syringe (piston, plunger) - no air cushion - for high density and very viscous - used for sampling in most discrete automated systems

Answer 28

positive displacement

Question 29

Calibration for semi-automatic micropipettors: how frequent?

Answer 29

6 mos

Question 30

Calibration for semi-automatic micropipettors: Gravimetric method- weight of ____ delivered; most accurate

Answer 30

distilled water (d= 1g/mL @20degC)

Question 31

Calibration for semi-automatic micropipettors: Spectrophotometric method - absorbance of ____ and ____ delivered

Answer 31

potassium dichromate para-nitrophenol

Question 32

Centrifuges: tubes attain a HORIZONTAL position during spinning, vertical when at rest

Answer 32

Horizontal or swinging bucket

Question 33

Horizontal or swinging bucket rpm?

Answer 33

3,000 rpm

Question 34

Centrifuges: tubes are at FIXED ANGLE when rotating

Answer 34

Fixed-angle or angle head

Question 35

Fixed-angle or angle head rpm?

Answer 35

7,000 rpm

Question 36

Centrifuge: used TO SEPARATE LAYERS of different specific gravities; usually refrigerated to counter the heat produced due to friction

Answer 36

ultracentrifuge

Question 37

Ultracentrifuge rpm?

Answer 37

10,000 rpm

Question 38

Centrifuge: for body fluid CELL COUNTS

Answer 38

cytocentrifuge

Question 39

Cytocentrifuge rpm?

Answer 39

200-2,000 rpm

Question 40

Units used to centrifuges

Answer 40

rpm Relative Centrifugal Force (g) Svedberg (s)

Question 41

Formula for converting rpm to RCF:

Answer 41

RCF(g) = 1.118x10^-5 x (r) in cm x rpm^2

Question 42

centrifuge QC parameters

Answer 42

timer, stopwatch speed - tachometer/strobe light thermometer (for refrigerated centrifuge)

Question 43

centrifuge QC frequency?

Answer 43

every 3 mos (quarterly)