Laboratory: HCG Flashcards

(65 cards)

1
Q

A glycoprotein hormone secreted by the trophoblast of the developing embryo that rapidly increases in urine or serum during early stages of pregnancy

A

Human Chorionic Gonadotropin (HCG)

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1
Q

Composition of HCG

A

Alpha and Beta subunit

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2
Q

The alpha subunit of HCG is similar to LH, FSH, and TSH. True or False?

A

True

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3
Q

Beta-hCG helps maintain the ovaries and stimulate the production of progesterone. True or False?

A

False, it helps maintain the corpus luteum

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4
Q

Detectable amount of Beta-hCG in normal pregnancy

A

25 mIU/mL

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5
Q

In normal pregnancy, Beta-hCG is secreted 8-10 days after implantation and approx. 2-3 days after conception. True or False?

A

False, it is secreted 2-3 days after implantation and 8-10 after conception

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6
Q

Peak levels of hCG is reached approximately ____ after the last ____ ____

A

2-3 months
Menstrual period

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7
Q

Serum hCG levels rise rapidly in early gestation. True or False?

A

True

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8
Q

This is the disappearance of HCG from blood after removal, characterized by implanted fertilized egg outside the womb

A

Ectopic Pregnancy

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9
Q

Monthly shedding of uterine lining in women when ovary is not fertilized within duration.

A

Menstruation

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10
Q

Normal cycle of menstruation before starting

A

28-day cycle

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11
Q

Menstruation can last for __

A

4-5 days

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12
Q

Occurs when a fertilized egg attaches to the lining of the uterus.

A

Pregnancy

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13
Q

Supports the developing embryo by thickening the wall of uterine lining

A

Progesterone

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14
Q

A test to detect the presence of Beta-hCG

A

Pregnancy Test

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15
Q

A male patient can possibly have a __ __ if __ on a pregnancy test

A

Malignant condition
Positive

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16
Q

Pregnancy Test can also determine trophoblastic diseases such as __, __, __ and __ (__)

A

Hydatidiform mole
Choriocarcinoma
Chorioepithelioma
Teratoma (Testicular tumor)

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17
Q

Urine sample collected for pregnancy test is ___

A

First morning

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18
Q

Intake of ____ and ___ should be restricted before pregnancy test

A

Fluid
Sedatives

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19
Q

Samples for pregnancy can be stored at _ to _ degrees Celsius for __ hours

A

2-8
48

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20
Q

If sample is refrigerated, place at room temperature for __ minutes before examination

A

30

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21
Q

If two lines appear in the C and T area of test kit, it is positive for pregnancy. True or False?

A

True

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22
Q

One line in the T area indicates negative for pregnancy. True or False?

A

False, one line in T area indicates that test is invalid

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23
Q

Negative for pregnancy is characterized by __ __ in _ area in test kit

A

One line
C area

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24
A recommendation for collection of new sample after 48 hours using another test kit happens when there is the presence of __ __
Faint band
25
False-positive result for pregnancy test can caused by increased ___, trophoblastic diseases and incomplete ___
Protein Abortion
26
Toxic urine can cause false negative result in pregnancy test. How do you detoxify urine?
Cellophane dialysis (addition of 0.1 NHCl)
27
Other causes of false-negative result besides toxic urine in pregnancy test:
Dilute urine Undetectable levels of HCG
28
Toxic urine can be caused by either __ or __
Bacterial contamination Excessive electrolyte content
29
Home-Based HCG Pregnancy Test Kit is based on what principle?
Enzyme Immunoassay
30
Urine samples for pregnancy testing should have an SG of ___ or higher
1.015
31
What is the source of anti-HCG?
Rabbit
32
In Home-Based HCG PT, anti-HCG is impregnated on the membrane strip. True or False?
True
33
This refers to the use of experimental animals to identify the presence of HCG
Biological Assay
34
Animal used in Ascheim-Zondek
Immature female mice
35
Animal used in Friedman
Mature virgin female rabbit
36
Animal used in Hogben
Female toad
37
Animal used in Galli-Mainini
Male Frog or Male Toad
38
Animal used in Frank-Berman
Immature female rats
39
Animal used in Kupperman
Female rat
40
Specific specie of female toad in Hogben
Xenopus laevis
41
Specific specie of male frog in Galli-Mainini
Rana pipiens Rana clamitans
42
Specific specie of male toad in Galli-Mainini
Bufo bufo Bufo americanus
43
Subcutaneous mode of injection is used in what biological assays?
Ascheim-Zondek Galli-Mainini Frank-Berman
44
Marginal ear vein injection is used in what test?
Friedman Test
45
Lymph sac injection is used in what test?
Hogben Test
46
Intraperitoneal injection is used in what test?
Kupperman Test
47
Positive result for Frank-Berman and Kupperman Test indicates what?
Ovarian hyperemia
48
Positive result for Hogben Test indicates?
Oogenesis
49
Positive result for Galli-Mainini Test indicates?
Spermatogenesis
50
Formation of hemorrhagic follicles and corpora lutea is characterized by positive result in what test?
Ascheim-Zondek
51
Hyperemic uterus and corpora hemorrhagica is characterized by positive result in what test?
Friedman Test
52
Types of Immunologic Assay for HCG:
Complement fixation Hemagglutination-inhibition Latex particle agglutination inhibition Direct agglutination Enzyme immunoassay
53
This test identifies the presence of progesterone
Pregnandiol Test
54
Positive Pregnandiol Test forms what color?
Deep orange or dark brown
55
Negative Pregnandiol Test forms what color?
Amber
56
This test identifies the presence of epinephrine
Iodine Test / Lugol's reaction test
57
Positive Iodine Test forms what color?
Deep orange or dark brown
58
How many weeks of waiting before results for Prostigmen Withdrawal Bleeding Test?
2 weeks
59
Bleeding in PWBT indicates?
Not pregnant Rationale: No bleeding indicates pregnancy
60
In Skin Colostrum Test, what is injected and where it is injected?
Breast milk Forearm
61
No swelling or redness in SCT indicates?
Pregnancy Rationale: Swelling indicates pregnancy
62
How many days of waiting before result in SCT?
3 days
63
How many tablets is given in Primodos Oral Test?
5 tablets
64
The tablets in Primodos Oral induce bleeding. No bleeding after 5 days indicates?
Pregnancy Rationale: Bleeding within 5 days indicates no pregnancy