LABORATORY SAFETY Flashcards
It includes all the information for contact tracing and the information
of the patient, travel information, symptoms of the patient, and chain
of custody
Client Information
Form or Test request
identifiers for the patient
Patient’s full name
Age/Birthday – most likely
Patient’s accession number
Must not have any leaks
− The swab should be included inside the vial
Sample Integrity
True or false
In RITM Interim guidelines for biosafety, there is unidirectional flow of
samples which is always be from clean to dirty.
True
Is a liquid that is usually used to preserve samples until it reaches
the laboratory.
Viral transport medium
Often called as the optimization or validation of methods
choice of analytical method
This is about how we analyze the sample itself.
analytical method
It is the process of removing the genetic material from the cells such as
those from viral or infected cells
Extraction
this is a test tube rack that can be placed in the
freezer and changes color when its cold. This is where the tubes with
samples are placed.
Isofreeze racks
Quality controls such as sterile/blank control, positive control, and
negative controls that are incorporated whenever we do molecular
analysis
Quality Assessment
Quality Assessment used to test whether the reagent is sterile
blank
Quality Assessment used to confirm if the procedure is able to detect
the positive or the targeting RNA
Positive control
negative should be not detected
Negative control
Storage temp requirement
-20°C
Laboratory Workflow
Pre analytical, analytical, post analytical
Are always written and always documented, similarly laws inside the
laboratory that must be followed by all the staff to ensure that all of your
results are released accurately, precisely, and of the best quality.
STANDARD OPERATING PROCEDURE
Encompasses all activities performed by laboratory personnel to
ensure reliability of test results
quality assurance
Quality control checks inside the laboratory, where we run samples with
negative and positive control, and a sterilized control
internal quality assurance
Wherein we test different laboratories with each other, Performed by a National Reference Laboratory (NRL)
external quality assurance
Given to a laboratory before they are given a license to operate or renew
their license
Tests the laboratory if it can work properly or the results are correct and
accurate
proficiency testing
is the entry point of all the laboratory workers and it is where
the lockers are located
Anteroom
Donning area on the right is usually the clean areas, it is also called
positive pressure areas
Donning area on the left is usually the dirty areas, it is also called
negative pressure areas
helps in the transfer of
samples or reagent from one section of the lab to another.
passbox
Receiving room, Specimen processing room, Specimen
Handling/Preparation room are done in
negative pressure area
Reagent preparation room, template adding room, PCR
room are in the
positive pressure areas
→ Potential that a chosen action will lead to an undesirable outcome
→ Probability of harm from occurring given a specific situation
a. hazard
b. risk
risk
→ Has the potential for causing harm
a. hazard
b. risk
hazard
Effect of uncertainty expressed by the combination of the consequences
of an event (including changes in circumstances) and the associated
likelihood of occurrence, where biological materials is the source of harm
a. biohazard
b.biorisk
c. biorisk management
d. risk
biorisk
Management system of part of a management system used to establish
biorisk management policies, objectives, and processes to achieve those
objectives
Biorisk Management System
Containment principles, technologies, and practices implemented to
prevent unintentional/accidental exposure to pathogens and toxins, or
their unintentional/accidental release.
a. Laboratory Biosafety
b. Laboratory Biosecurity
Laboratory Biosafety
Protects bad bugs from bud people
Laboratory Biosecurity
purpose of Biosecurity
- Protect users
- Protect those outside the lab
- Protect the environment
