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Microbiology - Mycology > Laboratory Techniques > Flashcards

Laboratory Techniques Flashcards

1
Q

detects fungal elements from skin, hair and nails

A

10-20% KOH

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2
Q

serve as preservative and stain

A

Lactophenol Cotton Blue

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3
Q

to demonstrate C. neoformans capsule in temporary mount

A

India ink / Nigrosin

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4
Q

to demonstrate chitin in cell wall

A

Calcofluor white stain

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5
Q

consider as the best stain in direct microscopy

A

Calcofluor white stain

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6
Q

Example of Temporary Mounts

A

10-20% KOH
Lactophenol Cotton Blue
India ink / Nigrosin
Calcofluor white stain

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7
Q

Example of Permanent Mounts

A

Periodic acid Schiff and Gomori’s Methanamine Silver
Mayer’s Mucicarmine
Fontana Masson and H&E Giemsa or Wright’s
Gram stain and
Acid fast/Kinyoun stain

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8
Q

Demonstrate fluorescens of fungal elements usually hair

A

Wood Lamp

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9
Q

detect fungi in histologic sections

A

Periodic acid Schiff and Gomori’s Methanamine
Silver

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10
Q

demonstrate C. neoformans in permanent mount

A

Mayer’s Mucicarmine

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11
Q

detects hyphal pigmentation

A

Fontana Masson and H&E

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12
Q

detects yeast within
mononuclear cells (ie. Histoplasma capsulatum)

A

Giemsa or Wright’s

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13
Q

Incubation temperature for fungi

A
  • 25-30degC or RT
  • Ambient air (most fungi grow within 2 weeks incubation)
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14
Q

Antimicrobial agents in
Fungal media

A
  • Chloramphenicol
  • Cycloheximide
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15
Q

prevent bacterial overgrowth

A

Chloramphenicol

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16
Q

prevent saprophytic fungi

A

Cycloheximide

17
Q

Culture media for Aspergillus

A

Czapek agar

18
Q

Culture media for dermpatophytes

A

Mycosel/Mycobiotic

19
Q

Culture media to stimulate chlamydospore formation of C. albicans

A

Cornmeal agar

20
Q

Culture media for primary recovery of saprobic and pathogenic fungi (Nocardia spp.)

A

Sabouraud’s Dextrose agar

21
Q

Culture media for C.
neoformans

A

Bird seed, Niger seed, Staib’s medium

Microbiology - Mycology (5 decks)

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