Learning Objectives Flashcards
(8 cards)
1
Q
Describe the differences in genes, genome structure, and organization between prokaryotes and eukaryotes
A
2
Q
- How does the Lac operon work?
- What are the important lessons from the Lac operon that apply to eukaryotic gene expression?
- How are regulatory sequences different and similar between prokaryotes and eukaryotes?
A
3
Q
Describe these methods
- Southerns
- Northerns
- Westerns
- autoradiography
- DNAse footprinting
- EMSA
- restriction enzymes and recombinant DNA
- cloning, vectors,
- cell culture,
- reporter assays,
- yeast‐two‐hybrid assays
A
4
Q
What is an enhancer?
A
5
Q
What is an insulator?
A
6
Q
What are specialized transcription factors? General transcription factors?
A
7
Q
What are DNA binding domains?
How do they recognize and interact with DNA?
What’s a palindrome?
A
8
Q
What is meant by differential gene expression?
What is the combinatorial model?
How does it relate to development?
A
