Lecture 2 Flashcards Preview

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Flashcards in Lecture 2 Deck (12):
1

How large or plasmids?

1000 base pairs

2

Do plasmids carry essential genes?

No

3

What do plasmids need in order to conjugate?

F factor

4

Where do anti-biotics come from?

bacteria

5

Describe the assay

 

DNAse I Footprinting

6

Describe the assay

 

 

Electrophoretic Mobility Shift Assay (EMSA)

7

 

 

What do you notice about the sequences which these DNA binding domains recognize?

Q image thumb

8

Describe the assay

 

Yeast two-hybrid assay

9

 

 

What were the two hypotheses for differential gene expression?

  1. Hypothesis #1: As cells become more restricted in their fate, they delete the genes they don’t need. 
     
  2. Hypothesis #2: Genes aren’t lost during development, but rather each cell becomes more and more restricted in its fate, expressing ultimately a specific subset of genes responsible for defining its specific function

10

Differential Gene Expression

 

 

Why not synthesize all the genes all the time at a moderate level?

11

 

 

What are the Principles of gene control?

12