Lecture 2 Flashcards
(12 cards)
1
Q
How large or plasmids?
A
1000 base pairs
2
Q
Do plasmids carry essential genes?
A
No
3
Q
What do plasmids need in order to conjugate?
A
F factor
4
Q
Where do anti-biotics come from?
A
bacteria
5
Q
Describe the assay
DNAse I Footprinting
A
6
Q
Describe the assay
Electrophoretic Mobility Shift Assay (EMSA)
A
7
Q
What do you notice about the sequences which these DNA binding domains recognize?
A
8
Q
Describe the assay
Yeast two-hybrid assay
A
9
Q
What were the two hypotheses for differential gene expression?
A
- Hypothesis #1: As cells become more restricted in their fate, they delete the genes they don’t need.
- Hypothesis #2: Genes aren’t lost during development, but rather each cell becomes more and more restricted in its fate, expressing ultimately a specific subset of genes responsible for defining its specific function
10
Q
Differential Gene Expression
Why not synthesize all the genes all the time at a moderate level?
A
11
Q
What are the Principles of gene control?
A
12
Q
A
