Flashcards in Lecture 2 Deck (12):
1
How large or plasmids?
1000 base pairs
2
Do plasmids carry essential genes?
No
3
What do plasmids need in order to conjugate?
F factor
4
Where do anti-biotics come from?
bacteria
5
Describe the assay
DNAse I Footprinting
6
Describe the assay
Electrophoretic Mobility Shift Assay (EMSA)
7
What do you notice about the sequences which these DNA binding domains recognize?
8
Describe the assay
Yeast two-hybrid assay
9
What were the two hypotheses for differential gene expression?
- Hypothesis #1: As cells become more restricted in their fate, they delete the genes they don’t need.
- Hypothesis #2: Genes aren’t lost during development, but rather each cell becomes more and more restricted in its fate, expressing ultimately a specific subset of genes responsible for defining its specific function
10
Differential Gene Expression
Why not synthesize all the genes all the time at a moderate level?
11
What are the Principles of gene control?
12