Lec 9

Question 1

What are the two main phases of glycolysis?

Answer 1

1. Preparative phase (consumes ATP)
2. Energy-harvesting phase (produces ATP)

Question 2

What is the overall energy yield of glycolysis per glucose molecule?

Answer 2

..

Question 3

What enzyme catalyzes the first step of glycolysis?

Answer 3

Hexokinase

Question 4

What is the “commitment step” of glycolysis?

Answer 4

The phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate by phosphofructokinase

Question 5

What enzyme converts glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate?

Answer 5

Glyceraldehyde-3-phosphate dehydrogenase

Question 6

In which step of glycolysis is NADH produced?

Answer 6

Step 6, catalyzed by glyceraldehyde-3-phosphate dehydrogenase

Question 7

Which steps in glycolysis produce ATP through substrate-level phosphorylation?

Answer 7

Step 7 (3-phosphoglycerate kinase)

Step 10 (Pyruvate kinase)

Question 8

What is the purpose of fermentation in anaerobic conditions?

Answer 8

To regenerate NAD+ so that glycolysis can continue

Question 9

What are two types of fermentation?

Answer 9

1. Lactic acid fermentation
2. Alcoholic fermentation

Question 10

Where does glycolysis occur in eukaryotic cells?

Answer 10

In the cytoplasm