[LEC] Rules of Maturation, Maturation Pools

Question 1

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND GRANULATION

Answer 1

INDIRECT
Cells lose their granules as they mature

Question 2

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND NUCLEUS

Answer 2

INDIRECT
Blast cells have the largest nucleus (3/8 to 7/8 of cell area)
and a SKANTY amount of cytoplasm

Question 3

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND CYTOPLASMIC BASOPHILIA

Answer 3

INDIRECT
DNA production decreases with maturation
DNA is made of amino ACIDS
Acid levels decrease
Attraction to blue stain also decreases

Question 4

WHAT CELL IS EXEMPTED FROM THE RULE OF MATURATION REGARDING THE BASOPHILIA OF THE CYTOPLASM

Answer 4

PLASMA CELL
Basophilia will not decrease as it ages ????

Question 5

WHAT IS THE COLOR OF A LEUKEMIC PATIENT’S BLOOD SMEAR AND WHAT RULE OF MATURATION IS RELATED TO IT

Answer 5

BLUE
In leukemia, there is an abundance of immature cells
Basophilia is a characteristic of immaturity in cells

Question 6

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND CHROMATIN

Answer 6

INDIRECT
As cells mature, the chromatin clumps more

Question 7

MATCH THE CELL TO ITS CELLULAR CHARACTERISTIC

1. Immature Cell
2. Mature Cell

A. Closed Chromatin
B. Open Chromatin

Answer 7

1. Immature — Open Chromatin
2. Mature — Closed Chromatin

Recall that chromatin clumps more are maturity is obtained by the cell

Question 8

[RULES OF MATURATION]
RELATIONSHIP OF CHROMATIN CLUMPING TO NUCLEUS SIZE

Answer 8

INDIRECT

Question 9

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND CHROMATIN STAINING

Answer 9

INDIRECT
The more aggregated the chromatin is, the darker they stain

Question 10

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND CELL SIZE

Answer 10

INDIRECT
Cellular components shrink, clump, and eventually cease to exist in the cell

Question 11

WHICH CELL IS EXEMPT FROM THE CELL MATURATION RULE ABOUT SIZE

Answer 11

MEGAKARYOCYTE
It is the largest mature cell in the BM

Question 12

WHAT IS THE LARGEST CELL IN THE BONE MARROW

Answer 12

MEGAKARYOCYTE

Question 13

WHAT IS THE LARGEST CELL IN THE PERIPHERAL CIRCULATION

Answer 13

MONOCYTE

Question 14

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND APPEARANCE OF NUCLEOLI

Answer 14

INDIRECT
Chromatin pattern becomes more coarse and condensed

Question 15

WHAT DO YOU CALL THE CELL WHEN ITS NUCLEUS IS VERY CONDENSED AND ITS PARACHROMATIN IS NO LONGER EVIDENT

Answer 15

PYKNOTIC

Question 16

APPEARANCE OF THE HEAVY CLUMPING OF NUCLEAR CHROMATIN

Answer 16

RASPBERRY-LIKE APPEARANCE

Question 17

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND THE APPEARANCE OF SPECIFIC GRANULES

Answer 17

INDIRECT
Granules become less prominent and decrease in size

Question 18

[RULES OF MATURATION]
RELATIONSHIP OF CELL MATURITY AND N:C RATIO

Answer 18

INDIRECT

Question 19

STAIN USED IN PBS

Answer 19

MODIFIED ROMANOWSKY STAIN
WRIGHT OR WRIGHT-GIEMSA STAIN

Question 20

THE DISAPPEARANCE OF NUCLEOLI PRECEDES THE CESSATION OF WHAT CELLULAR PROCESS

Answer 20

PROTEIN SYNTHESIS

Question 21

COLOR TRANSITION OF CELLS AS THEY MATURE

Answer 21

BLUE
GRAY-BLUE
SALMON PINK

Question 22

WHAT IS THE PRINCIPLE OF BASOPHILIA IN CELLS

Answer 22

DUE TO ACIDIC COMPONENTS THAT ATTRACT BASIC STAINS

Question 23

BASIC STAIN USED

Answer 23

METHYLENE BLUE

Question 24

WHY DOES ACIDOPHILIA INCREASE AS CELLS MATURE

Answer 24

THE ACCUMULATION OF HEMOGLOBIN
Active protein production diminishes as cells mature
Hemoglobin synthesis begins to occur
The proteins and hemoglobin mix
Protein synthesis stops, ribosomes are no longer present, and only hemoglobin remains

Question 25

FOUR TYPES OF GRANULES

Answer 25

NEUTROPHILIC — TAN OR PURPLE BASOPHILIC — BLUE EOSINOPHILIC — DEEP ORANGE TO RED AZUROPHILIC — PRIMARY GRANULES (??)

Question 26

[MATURATION POOLS] PRECURSORS ARE CAPABLE OF CELL DIVISION

Answer 26

MITOTIC POOL

Question 27

[MATURATION POOLS] CELLS NO LONGER DIVIDE

Answer 27

STORAGE POOL

Question 28

[MATURATION POOLS] CELLS ARE READY FOR MATURATION

Answer 28

STORAGE POOL

Question 29

OTHER TERM FOR STORAGE POOL

Answer 29

MATURATION POOL

Question 30

[MATURATION POOLS] CELLS ARE ACTIVELY DIVIDING

Answer 30

MITOTIC POOL

Question 31

[MATURATION POOL] SEGMENTERS IN CIRCULATION

Answer 31

CIRCULATING POOL

Question 32

[MATURATION POOL] CELLS FROM THIS POOL ARE STIMULATED TO MIGRATE TO THE TISSUES

Answer 32

CIRCULATING POOL

Question 33

[MATURATION POOL] CELLS STAY AND REST

Answer 33

MARGINATING POOL

Question 34

[MATURATION POOL] CELLS TEMPORARILY ADHERE TO THE EPITHELIAL CELLS

Answer 34

MARGINATING POOL PAVEMENTING POOL

Question 35

CELL SURFACE LECTINS THAT ATTACH CELLS TO THE ENDOTHELIAL CELLS

Answer 35

SELECTINS

Question 36

OTHER TERM FOR THE MARGINATING POOL

Answer 36

PAVEMENTING POOL

Question 37

[MATURATION POOL] CELLS ARE ABLE TO PERFORM PHAGOCYTOSIS

Answer 37

TISSUE POOL

Question 38

[MATURATION POOL] DIAPEDESIS OCCURS

Answer 38

TISSUE POOL

Question 39

WHAT IS DIAPEDESIS

Answer 39

THE EXIT OF CELLS FROM THE BLOOD VESSELS

Question 40

SUBSTANCES THAT INDUCE DIAPEDESIS

Answer 40

SELECTIN INTEGRIN

Question 41

DIRECTION OF CELL MIGRATION DURING DIAPEDESIS

Answer 41

ZIGZAG MANNER

Question 42

DIRECTION OF CELL MIGRATION DURING CHEMOTAXIS

Answer 42

MOVEMENT IS STRAIGHT FORWARD

Question 43

CELLULAR MOVEMENT TOWARD OR AWAY FROM A CHEMICAL STIMULUS

Answer 43

CHEMOTAXIS