LEC4: Lipids and the Plasma Membrane Flashcards Preview

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Flashcards in LEC4: Lipids and the Plasma Membrane Deck (50):
1

what are lipids?

molecules that're soluble in fat, typically contain either long hydrocarbon chains (eg fatty acids) or multiple linked aromatic rings (eg steroids)

store energy in the body 

main component of cell membranes 

play role in many signaling pathways

2

what are fatty acids?

how are they differentiated?

type of lipid

have long hydrocarbon chain (C-H) w/ a carboxyl group (polar, acidic, COO-) at 1 end 

differentiated by:

1) number, location of double bonds

2) length of hydrocarbon chain

3

saturated vs. unsaturated fatty acid?

saturated: no double bonds btwn C-H

unsaturated: has some double bonds btwn C-H

4

how are fatty acids named?

C:DΔ

# of carbon atoms:number of double bonds, Δ=positions of double bonds starting from COOH carbon

 

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5

what causes a bend in hydrocarbon chain of fatty acids?

double bonds in fatty acids are in cis configuration

double bond causes bend

6

numbering of oleic acid?

18:1Δ9

7

numbering of alpha-linolenic acid?

18:3Δ9, 12, 15

8

numbering of arachidonic acid?

20:4Δ5,8,11,14

9

what is glycerol? 

glycerol: 3-C sugar alcohol 

 

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10

how do glycerol + fatty acid come together? 

what do they form? function of that structure?

ester of glycerol + fatty acid = glycerolipid 

if all 3 OHs in glycerol are esterified, = triglyceride 

triglycerides are energy stores in cells, present as fat droplets

11

what are glycerophospholipids or phosphoglycerides?

glycerol-based phospholipids 

formed when 2 OH groups in glycerol are in etser linkages to fatty acids, 3rd is attached to a polar headgroup through a phosphate group

main component of plasma membranes 

 

12

structure of glycerophospholipids? 

2 OH groups of glycerol in ester linkages to fatty acids

3rd OH of glycerol attached to a polar head group thru a phosphate group 

backbone = phosphatidic acid 

most abundant examples have ethanolamine, choline, serine attached to phosphate group

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13

most common glycerophospholipids in cell membrane?

phosphatidylcholine

phosphatidylethanolamine

phosphatidylserine 

14

simplest glycerophospholipid?

phosphatidic acid 

has H as its X-group

 

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15

what is structure of cephalin? where is it found?

aka phosphatidyl-ethanolamine 

has good insulating properties, so found in white matter of brain, spinal cord 

has ethanolamine as X-group 

 

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16

structure of the plasma membrane?

lipid bilayer with hydrophobc fatty acid tails of phospholipds facing each other, polar head groups facing outward 

thus functions as hydrophobc barrier btwn interior of cell and external environment 

 

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17

what is the function of phosphatidylinositol?

phospholipid where X group is a sugar 

found on INNER LAYER of plsame membrane, where it binds molecules, gets phosphorylated, gets cleaved - so involved w/ specific cell-signaling pathways 

 

18

what is cardiolipin?

member of phosphatidylinositol family 

2 molecules of phosphatidic acid joined head-to-head by glycerol group

only found in inner mitochondrial membrane & chloroplasts of eukaryotes/prokaryotic membranes (NOT PLASMA MEMBRANE) where it transports electrons in e.t.c 

 

19

what is this molecule? 

where is it found?

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cardiolipin 

found in mitochondrial membrane

20

what is the structure of a sphingolipid? what does this family of membrane lipids include? 

sphingosine backbone w/ 2 long hydrocarbon chains, no phosphate group 

includes: ceramide, sphingomyelin, glycolipids

 

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21

what is the variable component of ceramide, in sphingomyelin, and in glycolipids?

ceramide: H

sphingomyelin: phosphocholine

glycolipids: mono- or oligosaccharide

22

what do defects in sphingolipid metabolism cause?

several types of inherited disorders called lysosomal storage diseases

23

glycolipids' main function?

energy storage in the body

have a carbohydrate group instead of phosphoryl group 

24

sphingosolipids' main function in the body?

significant component of membranes

25

function of sphingomyelin?

significant component of lipid bilayer, usually inner layer 

protects cell from mechanical and chemical interruption 

good insulator

 

26

ganglioside function?

store sugar

27

what is tay sachs?

lipid storage disorder caused by mutations in sphingolipid metabolism w/ hexosaminidase enzyme 

accumulation of gangliosides in nerve cells > becomes toxic to other pts of body > children die, by age 6

dx w/ white patch on the eye 

 

28

what is the structure of cholesterol?

what is its fxn in plasma membrane?

multi-ring structure (steroid nucleus), hydrocarbon tail, hydroxyl group (polar pt of structure!) 

increases impermeability of bilayer to small polar molecules, ie H2O

29

what is this? 

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cholesterol

see RIGID STRUCTURE makes cell plasma membrane very rigid

30

what is the nature of the distribution of lipids in the plasma membrane?

highly asymmetric

lipids' fxn determines if they're on inside or outside of membrane

31

which phospholipids are in which part of the plasma membrane, and why? 

cytosolic side: PI (b/c precursor for signaling molecs), PS (monitors cell viability), PE

extracellular side: PC, sphingomyelin, glycolipids 

in both monolayers: cholesterol 

32

what does the presence of PS on the outer monolayer of the plasma membrane indicate?

indication that cell is undergoing apoptosis b/c PS monitors cell viability, usually is localized to cytosolic side of bilayer

33

what is membrane permeable to?

gases (CO2, O2)

hydrophobic molecules, like hydrocarbons (benzene)

34

what is membrane slightly permeable to?

small polar molecules (H2O, ethanol)

35

what is plasma membrane impermeable to?

large polar molecules, charged species (glucose, amino acids, ions)

36

what is the effect of increaisng the concentration of cholesterol in a membrane?

it decreases the permeability of the membrane to small polar molecules like water 

b/c cholesterol is so rigid, does not allow passage 

37

why does aspirin work?

aspirin is a weak acid 

protonated form is uncharged

therefore can cross membrane by diffusion

38

what are the components of a plasma membrane?

ratio of these components?

both lipids and proteins

ratio of lipd to protein molecules is 50:1

39

what is meant by the "fluid mosaic" model of the plasma membrane?

because lateral diffusion in a membrane is so rapid, indicates that membrane is essentially a two-dimensional liquid 

fluid nature of the plasma membrane allows cells to change shape rapidly w/o being ruptured

40

different ways proteins can be associated w/ membranes?

1) transmembrane proteins: cross out-inside

2) monolayer-associated: bind only to 1 layer of lipid bilayer

3) lipid-linked: bind to lipid assoc w/ membrane, but do not cross it

4) protein-linked: bind to a transmembrane protein

41

what are the 2 types of transmembrane proteins that contain regions that span the membrane?

1) alpha-helical type: eukaryotes

2) beta type: beta barrel, in prokaryotes - gram-negative bacteria, cell wall of gram-positive bacteria; in euk, only in outer membrane of mitochondria (and chloroplast)

42

what are integral membrane proteins 

directly inserted into a lipid membrane or are covalently bound to other groups that're inserted in a membrane 

or only inserted into inner leaflet of plasma membrane

attached to membrane thru covalent linkage to a fatty acid, hydrocarbon tail, phospholipid attachment

43

what are peripheral membrane protines

those associated w/ membrane thru non-covalent interactions w/ other proteins

44

what is the structure of alpha-helical transmembrane proteins?

1) hydrophobic side chains that interact w/ lipid bilayer

2) positive side chains to residues that interact w/ phospholipid head groups 

3) positively charged residues in intracellular regions close to membrane, b/c inteact w/ particular lipidsin tehse regions 

4) polar sidechains for transmembrane residues that face a polar channel

45

what are 3 major classes of lipids found in the plasma membrane? 

how do their properties differ? 

1) glycerolipids, store energy via fat droplets

2) glycerophospholipids, major cell membrane components

3) sphingolipids, also membrane lipids, but do not contain phosphate group

46

arachiodonic acid is named 20:4Δ5, 8, 11, 14. what do these numbers designate?

numbers designate the position of double bonds w/ respect to COOH end of hydrocarbon tail 

47

why aren't triglycerides found in cell membranes?

all 3 of its OH groups are esterified, it does not have a polar head group, so it could not be a membrane component

48

what are some lipids that're differentially distributed in the plasma membrane? 

describe the significance of this observation 

asymmetric distribution in plasma membrane b/c of different function of each kind of lipid 

PS (monitors cell viability), PI (signaling molecule) are negative, PE is neutral, are on cytosolic side of bilayer

PC, sphingomyelin, glycolipids are on extracellular side of bilayer 

cholesterol is in both monolayers

49

what properties contribute to ability of molecules to cross membranes?

make up of membrane - so if only phospholipids or if also proteins 

also partical itself- permeable gas and hydrophobic molecules pass easily; small polar molecules pass mostly easily; large polar molecuels and charged species cannot pass

50

discuss different ways in which proteins are associated w/ membranes

proteins can be components of plasma membranes 

may be: transmembrane, monolayer-associated, lipid-linked, or protein-attached

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