Lecture 16 Flashcards
(8 cards)
Explain precipitation test
Precipitation test
eg. agar gel diffusion
soluble ag corsslinked by ab in samples which forms a precipitate
tube, plates or agar gel formats are used
steps
List the steps of a precipitin test
- serum from animal is placed on a well
- +ve control is placed on anther
- soluble ag goes on top 3rd
- formation of white line precipitin bw sample well, if there is a well (Hole) this is a positive test
For Immunofluorescent Ab test what does it detect and list the steps
Immunofluorescent Ab test used to detect ag or ab steps for ab detection ag in section, smear, culture -Ab in serum binds ag -bound ab detached by binding to fluorescence conjugated anti-globulin it will glow and be seen
For Immunochromatography -pen side test
what does it detect and list the steps
Immunochromatography -pen side test
used to detect ag or ab
for ag detection
sample containing Ag flows through porous strip containing dried labeled ab and forms immune complexes, ab is labelled with gold, fluid with immune complexes flow through the detection zone and binds immobilising abs
for positive results there will be a colour change
For Immunohistochemistry what does it detect and explain the steps
Immunohistochemistry
detects Ag in situ in tissues
shows presentce of T cells in lamina propria and epithelium of canine duodenum
tissue fixed and thin sections are placed on the microscope slides
cells binding the ab recognise canine CD3 are exposed to peroxidase- labelled specific anti globulin
presence of peroxidase will turn brown
Explain Flow cytometry
Flow cytometry
can tell you about the size and the granularity and internal complexity
-fluorescent labels cell surface or intraceullular ag using fluorescent ab
multiple ab can be used to stain multiple markers on a single cell
can determine relative % of immune cells
When is immunodiagnostic used
4
immunodiagnostic used diagnosis of -infectious disease, ab or ag detection -allergy and hypersensitivity -immune deficiencies -autoimmunity -tumour immunology
What diagnosis test can be done for allergies
diagnosis test for Allergy -serum ELISA -immunochromatography- looking for IgE can be done through skin or intradermal skin testing (allergens are injected into the skin and the skin will react with variable redness )
