Flashcards in Lecture 20 Deck (11):
Conservation biologists have two challenges:
- Which ones are boys, which ones are girls?
- How related are they?
To determine sex for each bird:
- Use a W-chomosome marker (2 bands girl, one band boy)
Screen micro satellite markers for each bird:
- Determine relatedness based on microsatellite data
- Negative result means they are the least related
Based on this data we can select the least related pairs to breed:
- A breeding program is designed to minimise inbreeding
Genetic rescue of the Florida panther (Puma concolour coryi):
- At brink of extinction in 1995 (Nc = 26)
- Low genetic diversity based on microsatellite data
- Many signs of inbreeding depression (kinked tails, poor semen quality, many parasites
How did they avoid extinction?
- Introduced 8 female cougars from Texas into the population of 26 individuals
What was the result of the introduced females?
- They thrived!
- Monitoring of the population showed great admixture of genetic material
What did they use as the genetic markers to monitor the cat populations?
- They used simple tandem repeats (STR) from the cat genome project
How effective was the program?
- The number of individuals from 26 to 102.
- The effective population has doubled
- Kinked tails and testicle problem dropped significantly
- The heterozygosity increased a lot!
Genetic rescue of Scandinavian grey wolf (Canis lupus):
- Mitochondrial (MtDNA) marker (female tracking)
- Y-chromosome marker (male tracking)
- Nuclear markers (SSRs)
- One male immigrant increased the population size a lot and also increased the level of heterozygosity