Lecture 21 - T Cell Trafficking Flashcards Preview

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Flashcards in Lecture 21 - T Cell Trafficking Deck (39):
1

Definition of naive T cells

Fully-developed T cells found in lymphoid organs that have not been activated by cognate interaction

2

Time for a naive T cell to make a full circuit

~24 hours

3

Naive T cell circulation circuit
1)
2)
3)
4)
5)

1) Lymph node
2) Enters efferent lymphatics
3) Efferent lymphatics empty into thoracic duct
4) Thoracic duct returns lymph to blood
5) Enter lymph nodes through HEV

4

Where can naive T cells never be?

Afferent lymphatics leading to tissue, tissue

5

Area of an artery with the least shearing force

Marginal pool (edges of artery)

6

What is tissue-specific migration of T cells called?

Homing

7

Do selectins or integrins bind with higher affinity?

Integrins

8

T cell surface molecule used for rolling

L-selectin

9

T cell surface molecule used for adhesion to endothelial wall

LFA-1

10

Another name for L-selectin

CD62-L

11

What is CD62-L?

L-selectin

12

What does L-selectin bind to?

Addressins

13

Addressin to HEV

GlyCAM-1

14

Addressin to endothelial cells

CD34

15

Addressin to endothelial cells in gut-associated lymphoid tissues

MadCAM-1

16

Where is GlyCAM-1 used as an addressin?

HEV

17

Where is CD34 used as an addressin?

Endothelial cells

18

Where is MadCAM-1 used as an addressin?

Endothelial cells in gut-associated lymphoid tissue

19

Chemokines associated with T cell diapedesis

CCL21, CXL12

20

Integrins
1)
2)
3)

1) Bind tightly to ligands
2) Signal through conformational changes
3) Large family of molecules, alpha and beta subunits

21

What does LFA-1 bind to?

ICAM-1

22

ICAM-1 minding molecule

LFA-1

23

What stimulates a naive T cell to become more adhesive to endothelial walls?

T cell hitting arterial wall causes conformational change in adhesion molecules

24

What do naive T cells always express at a high level?

CCR7

25

Where in a lymph node do HEV lie?

Paracortex

26

How do naive T cells enter lymph nodes?
1)
2)
3)

1) Through HEV, into paracortex
2) Attracted to DCs with CCL19, CCL21 with CCR7
3) Probe DCs for antigen

27

Name of cluster of adhesion and costimulatory molecules between a T cell and a DC

Immunological synapse

28

What is an immunological synapse?

Collection of integrins, MHC/TCR, CD28/CD80/86 and other molecules between T cell and DC

29

How long do cognate T cells and DCs bind together for?

Hours

30

Distribution of microclusters in naive T cell

Evenly distributed

31

What happens to mice without LFA-1?

No T cell activation
Immunological synapse is improperly formed, even if cognate with DC
LFA-1 can't cause tight binding of DC and T cell

32

State of IL-2 receptor in naive T cell

Only beta and gamma subunits present

Weakly respond to IL-2

33

State of IL-2 receptor in activated T cell

Alpha, beta and gamma subunits present

Strongly respond to IL-2

34

T cell function that requires IL-2

Entry into the cell cycle (division)

35

T cell retention signal in lymph node

CD69

36

T cell exit signal from lymph node

S1P1

37

How does S1P1 stimulate T-cells leaving lymph node?

S1P1 very strongly present in efferent lymph
When a T cell is activated, it begins expressing a lot of S1P1 receptor

38

Phenotype of T cells being retained in a lymph node

Highly express CD69 receptor, weakly express S1P1 receptor

39

Can a T cell strongly express both CD69 and S1P1?

No