Lecture 25 Flashcards

(5 cards)

1
Q

What is Transgenesis

A

Engineering a multicellular organism by adding in “foreign” DNA

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2
Q

How do we know if a gene variant is pathogenic?

A

We can damage, or modify, the gene we are interested in by
genetically modifying an organism or cell line.

By examining the organism, or its offspring, we should be able to
work out what the gene normally does.

One way to do this is via CRISPR-Cas9

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3
Q

Describe Targeted mutation with CRISPR-Cas9

A

Cas9 protein and Guide RNA with a complementary sequence that can bind to a target gene

Cas9 enters the nucleus and
finds the target sequence in
the genome that matches
guide RNA

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4
Q

How does cutting a gene with targeted mutation help scientists

A

They can disable a gene to study its normal function

If the target gene has a mutation it can be repaired

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5
Q

Describe what happens once the CRISPR-Cas9 is inside the cell

A

Cas9 enters the nucleus and
finds the target sequence in
the genome that matches
guide RNA

Cas9 makes double stranded
break in DNA at target site

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